Cells were harvested and red blood cells were lysed. Cells were stained with various Abs for 30 min and subjected to 12-color LSRII (BD Biosciences, San Jose, CA) for flow cytometric analysis, or FACS Aria II (BD Biosciences, San Jose, CA) for cell sorting. Results were analyzed by Flowjo7 data analysis software (FLOWJO, LLC Ashland, OR).
Flowjo7 data analysis software
Manufactured by FlowJo
FlowJo7 is a data analysis software designed for processing and visualizing flow cytometry data. The software provides tools for data management, gating, and statistical analysis of flow cytometry samples.
Automatically generated - may contain errors
Lab products found in correlation
Facsaria 2, by BD (1 mentions)
12 color lsrii, by BD (1 mentions)
Facsaria 3 sorter, by BD (1 mentions)
Anti cd45 apc, by Thermo Fisher Scientific (1 mentions)
Anti cd105 pe, by Thermo Fisher Scientific (1 mentions)
Anti sca 1 pe cy7, by Thermo Fisher Scientific (1 mentions)
Facscanto 2 cytometer, by BD (1 mentions)
2 protocols using flowjo7 data analysis software
1
Multiparameter Flow Cytometry Analysis
2
Multicolor Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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APC-anti-CD45, PE-anti-CD105 and PE-cy7-anti-Sca1 antibodies (Abs) are purchased from eBioscience. Callus and BM cells are stained with various fluorescein-labeled Abs and subjected to flow cytometric analysis using a Becton-Dickinson FACSCanto II Cytometer, according to the manufacturer’s instructions. Results are analyzed by Flowjo7 data analysis software (FLOWJO, LLC Ashland, OR). For cell sorting, BM cells from Nestin-GFP mice are sorted using a Becton-Dickinson FACSAria III sorter, according to the manufacture’s instructions [29 ].
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