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No 1.5 glass coverslip

Manufactured by Avantor

No. 1.5 glass coverslip is a thin, transparent, and flat piece of glass used in various laboratory applications. It serves as a protective cover for samples placed on microscope slides, allowing for clear observation and analysis.

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2 protocols using no 1.5 glass coverslip

1

Fluorescence Imaging of E. coli Cells

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A total of 1 ml culture of the E. coli strain to be imaged was grown from an overnight culture till OD600 = 0.1–0.2. It was then chilled on ice followed by centrifugation at 6000 × g, 4 °C for 1 min to form a cell pellet. Then they were washed with ice-cold 1× PBS twice and resuspended in 100 μl 1× PBS.
In all, 1.5% (w/v) agarose gel was prepared by dissolving agarose in 1× PBS. A few μl cell suspension was sandwiched between a No. 1.5 glass coverslip (VWR) and a thin slab of the agarose gel. The sample was then imaged.
The epifluorescence images were acquired by a Nikon Ti Eclipse microscope (Nikon Instruments, Inc.) using an oil immersion objective (1.46 NA, 100×), which spans an area of ~133 × 133 μm2 for DIC (no filter, autofluorescence) and fluorescence imaging (Ex 480–500 nm, Em 509–547 nm, exposure time 200 ms). The images were acquired using an EMCCD camera (Andor). They were processed using the NIS-Element AR software (Nikon Instruments, Inc.).
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2

Fluorescence Imaging of E. coli Cells

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1 ml culture of the E. coli strain to be imaged was grown from an overnight culture till OD600 = 0.1-0.2. It was then chilled on ice followed by centrifugation at 6000 g, 4 ºC for 1 minute to form a cell pellet. Then they were washed with ice-cold 1X PBS twice and resuspended in 100 µl 1X PBS.
1.5% (w/v) agarose gel was prepared by dissolving agarose in 1X PBS. A few µl cell suspension was sandwiched between a No. 1.5 glass coverslip (VWR) and a thin slab of the agarose gel. The sample was then imaged.
The epifluorescence images were acquired by a Nikon Ti Eclipse microscope (Nikon Instruments, Inc.) using an oil immersion objective (1.46 NA, 100X) which spans an area of around 133x133 µm 2 for DIC (no filter, autofluorescence) and fluorescence imaging (Ex 480-500 nm, Em 509-547 nm, exposure time 200 ms). The images were acquired using an EMCCD camera (Andor). They were processed using the NIS-Element AR software (Nikon Instruments, Inc.).
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