Korean chestnuts (Castanea crenata) were purchased from local markets. They were peeled, sealed in a vacuum plastic bag, and stored at 4 °C for subsequent use. Before use, the chestnuts were covered with a layer of wet cotton and allowed to germinate for 3 days at 18 °C.
HiTrap DEAE Sepharose Fast Flow, HiTrap Q Sepharose Fast Flow, and HiPrep Sephacryl S-100 HR were purchased from Cytiva (Uppsala, Sweden). Trizma® base (≥ 99.9%), diethyl ether (≥ 99.9%), p-nitrophenyl palmitate (p-NPP), sodium dodecyl sulfate (SDS), isooctane (IOT), tributyrin, tricaproin, tricaprin, trilaurin, tripalmitin, and triolein (≥ 99.0%) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Ammonium sulfate (≥ 99.5%), Triton X-100, sodium chloride (≥ 99.5%), and agar were purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan). Boric acid, potassium chloride, and oleic acid were obtained from Duksan Pure Chemicals Co., Ltd. (Ansan, Gyeonggi-do, Korea). The Costar® 96-well microplates (clear wall, clear bottom) used for the fluorometric assay were purchased from Corning Co. (Corning, NY, USA). All other chemicals were of analytical grade and were used without further purification.
HiTrap DEAE Sepharose Fast Flow, HiTrap Q Sepharose Fast Flow, and HiPrep Sephacryl S-100 HR were purchased from Cytiva (Uppsala, Sweden). Trizma® base (≥ 99.9%), diethyl ether (≥ 99.9%), p-nitrophenyl palmitate (p-NPP), sodium dodecyl sulfate (SDS), isooctane (IOT), tributyrin, tricaproin, tricaprin, trilaurin, tripalmitin, and triolein (≥ 99.0%) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Ammonium sulfate (≥ 99.5%), Triton X-100, sodium chloride (≥ 99.5%), and agar were purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan). Boric acid, potassium chloride, and oleic acid were obtained from Duksan Pure Chemicals Co., Ltd. (Ansan, Gyeonggi-do, Korea). The Costar® 96-well microplates (clear wall, clear bottom) used for the fluorometric assay were purchased from Corning Co. (Corning, NY, USA). All other chemicals were of analytical grade and were used without further purification.