Alexa fluor 568 conjugated goat anti rabbit igg h l

The following antibodies used in this study were obtained commercially: anti-HA rat monoclonal antibody (clone 3F10) (#11867423001; Roche Diagnostics, Mannheim, Germany); Alexa 488-conjugated goat anti-rat IgG(H+L) (#ab150165; Abcam, Cambridge, UK); anti-human c-Kit (K963) rabbit IgG (#18101; IBL, Fujioka, Japan); Alexa Fluor 488-conjugated goat anti-mouse IgG(H+L) (#A11029); Alexa Fluor 568-conjugated goat anti-rabbit IgG(H+L) (#A11011; Thermo Fisher Scientific, Waltham, MA, USA); anti-Pmel mouse monoclonal antibody (anti-human melanosome, clone HMB45) (#M0634; Dako, Carpinteria, CA, USA); anti-desmoplakin 1 guinea pig polyclonal, serum (#DP-1; PROGEN, Wayne, PA, USA); and Alexa Fluor 647 goat anti-guinea pig IgG (H+L) highly cross-absorbed (#A-21450; Thermo Fisher Scientific).

PP2A and rab7 antibodies were purchased from Cell Signaling (Danvers, USA). NKA α antibody, HPRT antibody, ANP antibody, 3-nitrotyrosine antibody, tubulin antibody, goat anti-rabbit and goat anti-mouse secondary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, USA). NKA α1 antibody (464.6, ab7671) was purchased from Abcam (Cambridge, UK). Alexa Fluor 568 conjugated goat anti-rabbit IgG (H + L), Alexa Fluor 488 conjugated goat anti-mouse IgG (H + L), FITC-conjugated wheat germ agglutinin were from Invitrogen Corporation (Carlsbad, USA). The McK1 antibody was a generous gift from Dr. K. Sweadner (Massachusetts General Hospital, MA). SensoLyte® FDP Protein phosphatase assay kit was from AnaSpec, Inc (AnaSpec, Inc, Japan). Primers for qPCR were from Integrated DNA Technologies. Collagenase (type I) was from Worthington Biochemical. DMEM medium, DMEM/F12 medium and fetal bovine serum were from HyClone. All other reagents were purchased from Sigma Chemical Company (St. Louis, USA).