Plasma triglycerides (TG) were measured with an enzymatic colorimetric commercial assay kit (ETGA-200 and EGLY-200, BioAssay Systems). Total cholesterol, combined low-density lipoprotein cholesterol (LDL-C), and very low density lipoprotein cholesterol (VLDL-C), as well as high-density lipoprotein cholesterol (HDL-C), were measured using an enzymatic colorimetric commercial assay kit (EHDL-100, BioAssay Systems). In the animals undergoing CEUMI, plasma HbA1c was measured using a sandwich enzyme-linked immunosorbent commercial assay kit (E4657-100, BioVision), and nonfasting plasma glucose was measured using mouse glucose assay kit (#81692, Crystal Chem) in blood drawn after the imaging studies. In a separate group of animals (n = 20 on liraglutide treatment and n = 20 on vehicle treatment), whole-blood HbA1c was measured at baseline, day 44, and day 80 in a 5-μL full blood sample taken from the tip of the tail by puncturing the capillary bed with a lancet, using a heparinized capillary tube to sample the blood. The capillary tube was then shaken into 500 μL of Hitachi Hemolyzing Reagent and measured in a Hitachi 912 autoanalyzer (Roche A/S Diagnostics), according to the manufacturer’s instructions. Assays were performed in duplicates according to manufacturer’s protocol with the exception of whole-blood HbA1c assays, which were done as single measurements.
Ehdl 100
Manufactured by BioAssay Systems
Sourced in United States
The EHDL-100 is a compact and versatile lab equipment designed for electrochemical analysis. It features a high-sensitivity potentiostat and galvanostat for performing a wide range of electrochemical measurements, including cyclic voltammetry, chronoamperometry, and electrochemical impedance spectroscopy.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using ehdl 100
1
Plasma Lipid and Glycemic Biomarkers
2
Blood Cholesterol Level Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice fasted overnight were euthanized with CO2, and whole blood was collected in a 1.5 ml tube. Whole blood was allowed to clot for 45 min at RT and centrifuged at 1,500 × g for 30 min at 4°C. The levels of cholesterol (high- and low-density lipoprotein, HDL and LDL, respectively) were determined using cholesterol assay kits (Cat#EHDL-100, Bioassay System, USA).
3
Comprehensive Metabolic Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood levels of glucose were quantified with Precision Xtra Glucose Monitor (#179837, Bound Tree Medical, LLC, Chicago, IL). Serum levels of insulin were determined with an ELISA kit (#90080, Crystal Chem USA, Downers Grove, IL). Serum levels of total cholesterol, high-density lipoprotein (HDL) cholesterol, and low-density lipoprotein (LDL)/very-low-density lipoprotein (VLDL) cholesterol were quantified with HDL and LDL/VLDL assay kit (EHDL-100, Bioassay Systems). Serum levels of free fatty acids were analyzed with EnzyChrom™ Free Fatty Acid Assay Kit (EFFA-100, Bioassay Systems). Serum levels of β-hydroxybutyrate were quantified with a colorimetric assay kit (#700190, Cayman Chemical, Ann Arbor, MI).
4
Enzymatic Biomarker Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Enzymatic colorimetric kits were used to determine serum TC (995280/QCA, Barcelona, Spain), HDL-cholesterol (HDL-c) and VLDL-cholesterol + LDL-cholesterol (VLDL-c + LDL-c) (EHDL-100/Bioassay Systems, California, USA), triglycerides (992330/QCA, Barcelona, Spain), glucose (992330/QCA, Barcelona, Spain) and nonesterified free fatty acids (NEFAs) (WAKO, Neuss, Germany). To analyse the levels of 17β-E2 at baseline and progesterone at the end point, the ELISA E2 kit (CSB-E05110r/CUSABIO, Houston, USA) and the ELISA progesterone kit (CSB-E07282r/CUSABIO Houston, USA) were used, respectively. Circulating insulin levels were measured using a rat/mouse ELISA kit (10-1250-01/MERCODIA, Upssala, Sweden). Serum leptin levels were determined with a rat ELISA kit (EZRL-83K/Millipore, Barcelona, Spain), and serum adiponectin levels were quantified by a Rat Total Adiponectin/ Acrp30 Quantikine ELISA kit (RRP300/R&Dsystems, Minnesota, USA). To quantify the circulating levels of the biomarkers of bone turnover procollagen I amino-terminal propeptide (PINP), osteocalcin (OC) and carboxy-terminal telopeptide of type I collagen (CTX), the PINP ELISA kit (AC-33F1, IDS, UK), the osteocalcin ELISA kit (AC-12F1, IDS, UK) and the CTX (AC-06F1, IDS, UK) were used, respectively.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!