Legend max β amyloid x 42 elisa kit

Human Aβ1–40 and Aβ1–42 concentrations were measured using commercially available ELISA kits (IBL International, Hamburg, Germany) according to the manufacturer’s protocol. Briefly, soluble or insoluble fractions were diluted 1:50 or 1:200, respectively, then added to wells coated either with anti-human Aβx–42 (44A3) or Aβx–40 (1A10) and incubated overnight at 4 °C. Wells were washed and incubated with HRP-conjugated anti-human Aβ1–x (82E1) for 1 h. After final washing steps, TMB solution was added as a substrate. The reaction was stopped with 1N H₂SO₄. Optical density (OD) values were measured at 450 nm on a microplate reader (Anthos 2010). For total Aβx–40/42 levels including N-terminally truncated peptides, samples were diluted 1:10 and the ELISA was performed according to the manufacturer’s instructions of LEGEND MAX™ β‐Amyloid x‐40 ELISA Kit (Biolegend, Cat. no.: 842301) and LEGEND MAX™ β‐Amyloid x‐42 ELISA Kit (Biolegend, Cat. no.: 842401).