Beibrich scarlet acid fuchsin solution

Manufactured by Merck Group

Beibrich Scarlet-Acid Fuchsin Solution is a laboratory reagent used for staining purposes in various scientific applications. It is a mixture of dyes, including Beibrich Scarlet and Acid Fuchsin, which can be utilized for specific staining techniques. The solution provides a distinct color and is commonly used in various microscopy and histological procedures.

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Lab products found in correlation

Aniline blue solution, by Merck Group (2 mentions) Histomount, by National Diagnostics (1 mentions) Digital image hub, by Leica (1 mentions) Weigert s haematoxylin solution, by Merck Group (1 mentions) Bouin s solution, by Merck Group (1 mentions) Phosphomolybdic phosphotungstic acid, by Merck Group (1 mentions)

Spelling variants of this product

Acid fuchsin (25 citations) Acid fuchsin solution (2 citations)

2 protocols using beibrich scarlet acid fuchsin solution

Quantifying Myocardial Infarct Size

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Masson’s trichrome staining was performed in order to visualise the infarct.29 Hearts were sectioned into 5 sets of slides, 10 slides per set. Sections were fixed for 1 h in 4% PFA followed by overnight incubation in Bouin’s solution (HT10132, Sigma), both at room temperature. Slides were washed in tap water, and nuclei were labelled with Weigert’s Haematoxylin solution (HT1079, Sigma). Cytoplasm staining was achieved by staining with Beibrich Scarlet-Acid Fuchsin Solution (HT151, Sigma) followed by an incubation in phosphotungstic/phosphomolybdic acid solution with ddH2O in a 1:1:2 solution respectively. Slides were incubated in Aniline Blue Solution (b8563, Sigma), washed in ddH2O and placed in 1% glacial acetic acid. Sections were dehydrated via an EtOH gradient washed in Histoclear (HS-202, National Diagnostics) and mounted using Histomount (HS-103, National Diagnostics). Each set of slides from each individual heart were imaged and analysed using the Leica Digital Image Hub. The left ventricular area was calculated by measuring the epicardial area and subtracting the endocardial area. The infarct area was then measured and the percentage of left ventricle that was infarcted was calculated as a percentage of the total left ventricle.

Redgrave R.E., Dookun E., Booth L.K., Camacho Encina M., Folaranmi O., Tual-Chalot S., Gill J.H., Owens W.A., Spyridopoulos I., Passos J.F, & Richardson G.D. (2023). Senescent cardiomyocytes contribute to cardiac dysfunction following myocardial infarction. NPJ Aging, 9(1), 15.

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Lung Tissue Processing and Staining

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Lung tissues were preserved in fixative 10% buffered formalin at room temperature. After 1 week formalin was replaced with 70% ethanol and stored at room temperature until processing. All samples were submitted and processed with hematoxylin and eosin and trichrome staining by the IU Histology core facility. Fixed samples were processed for paraffin embedding in blocks and cut by rotary microtome into 5 μm thick sections. Sections were mounted on glass slides and stained. For H&E staining, slides were stained following the LINISTAIN GLX linear stainer method with slides undergoing washes in xylenes, ethanol, Harris’ hematoxlin (Surgipath), 70% Ethanol + HCl, 0.3% ammonium hydroxide + water, and eosin (Surgipath). For Trichrome staining, deparaffinized slides were fixed in Bouin’s solution (37% Formaldehyde + Picric acid + Glacial acetic acid) and microwaved on high for 1 minute. Slides were stained with Gill’s No. 3 Hematoxylin (Surgipath), Scott solution (0.2% sodium bicarbonate + 2% magnesium sulfate), Beibrich Scarlet-Acid Fuchsin Solution (Sigma), Phosphotungstic / Phosphomolybdic Acid (Sigma), and Aniline Blue solution (Sigma).

Ulrich B.J., Kharwadkar R., Chu M., Pajulas A., Muralidharan C., Koh B., Fu Y., Gao H., Hayes T.A., Zhou H.M., Goplen N.P., Nelson A.S., Liu Y., Linnemann A.K., Turner M.J., Licona-Limón P., Flavell R.A., Sun J, & Kaplan M.H. (2022). Allergic airway recall responses require IL-9 from resident memory CD4+ T cells. Science immunology, 7(69), eabg9296.

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