Methyl β d glucoside

The restriction endonucleases, pfu turbo polymerase, and T4 DNA ligase, used in the cloning of ycjQ, ycjR, and ycjS, were purchased from New England BioLabs. The PCR cleanup and gel extraction kits were bought from Qiagen. The plasmid miniprep kit, buffers, phenylmethylsulfonyl fluoride (PMSF), DNase I, and Chelex resin were obtained from Sigma-Aldrich. Isopropyl-β-d-thiogalactopyranoside (IPTG), NAD⁺, NADH, NADP⁺, and NADPH were purchased from Research Products International Corporation. All isotopically-labeled derivatives of d-glucose (1) and d-gulose (8) were purchased from Omicron Biochemicals. Other carbohydrates were obtained from Carbosynth, with the exception of methyl α-d-glucoside (2) and methyl β-d-glucoside (3), which were purchased from Sigma Aldrich.