Dictyophora indusiata was purchased in Nanping Shengli Market. After the Dictyophora indusiata is air-dried, it is fully ground into powder by a high-speed pulverizer. DPPH (1,1-diphenyl-2-picroyl), ABTS (total antioxidant activity kit), PMS, NATB were purchased from Sigma Company in the United States (America). EDTA, trichloroacetic acid, ascorbic acid, Tris-hydrochloric acid buffer, H2O2 absolute ethanol, chloroform, sodium chloride, concentrated sulfuric acid, chloroform, n-butanol were purchased from Shanghai Chemical Reagent Factory (Shanghai, China). Methanol, trifluoroacetic acid, sodium hydroxide and sodium acetate trihydrate were purchased from sigma (Sigma–Aldrich, St. Louis, MO, USA). The chemicals and solvents used in this study were of analytical grade.
Chloroform
Manufactured by Shanghai Chemical Reagent
Sourced in China
Chloroform is a colorless, dense, and volatile liquid chemical reagent. It has the molecular formula CHCl3 and is commonly used as a solvent in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Milli q system, by Merck Group (2 mentions)
Toluene, by Shanghai Chemical Reagent (1 mentions)
Maleic anhydride, by Shanghai Chemical Reagent (1 mentions)
Epichlorohydrin, by Shanghai Chemical Reagent (1 mentions)
Citric acid, by Shanghai Chemical Reagent (1 mentions)
1 4 dioxane, by Shanghai Chemical Reagent (1 mentions)
P toluenesulfonic acid, by Shanghai Chemical Reagent (1 mentions)
Na2so4, by Shanghai Chemical Reagent (1 mentions)
Hydrochloric acid (hcl), by Shanghai Chemical Reagent (1 mentions)
Acetone, by Shanghai Chemical Reagent (1 mentions)
Sodium hydroxide (naoh), by Shanghai Chemical Reagent (1 mentions)
Isopropanol, by Shanghai Chemical Reagent (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Bca protein quantification kit, by Abcam (1 mentions)
Concentrated sulfuric acid, by Shanghai Chemical Reagent (1 mentions)
Ascorbic acid, by Shanghai Chemical Reagent (1 mentions)
7 ethyl 10 hydroxycamptothecin sn 38, by Merck Group (1 mentions)
Methanol, by Shanghai Chemical Reagent (1 mentions)
8 protocols using chloroform
1
Antioxidant Potential of Dictyophora indusiata
2
Plasma miRNA Extraction from EDTA-Preserved Blood
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Blood was drawn from the above-mentioned subjects recruited into this study. About 1 ml of peripheral whole blood were collected from each individual into free-enzyme-containing ethylenediaminetetraacetic acid (EDTA) tubes. Following the protocol provided by the producers, the blood was collected into EDTA tubes for centrifugation at 12,000 g for 10 min at 4 °C. Finally, the precipitate was collected into 1.5 ml Eppendorf (EP) tubes and stored at −80 °C for further analysis.
After the preparation of the plasma, the miRNAs were extracted using the TRIZOL method, following the producers’ protocol. We add 0.75 m L of TRIZOL reagent and 0.2 m L of chloroform (Shanghai Chemical Reagent Co., Ltd, China) to the samples and mix well, let it stand for 3–5 min; centrifuge for 10 min at 4 °C and 12,000 g centrifugal force; aspirate the supernatant and transfer it to another EP tube, then add 0.5 m L of isopropanol and mix well. Incubate at −20 °C overnight; centrifuge again at 4 °C and 10,000 g for 10 min, discard the supernatant, then add 1 m L of 75% ethanol, mix well, and continue centrifugation at 4 °C and 10,000 g for 10 min; then air dry at room temperature, add 30 μL of DEPC-treated dd H2O water to dissolve the RNA, and store at −80 °C. Storage.
After the preparation of the plasma, the miRNAs were extracted using the TRIZOL method, following the producers’ protocol. We add 0.75 m L of TRIZOL reagent and 0.2 m L of chloroform (Shanghai Chemical Reagent Co., Ltd, China) to the samples and mix well, let it stand for 3–5 min; centrifuge for 10 min at 4 °C and 12,000 g centrifugal force; aspirate the supernatant and transfer it to another EP tube, then add 0.5 m L of isopropanol and mix well. Incubate at −20 °C overnight; centrifuge again at 4 °C and 10,000 g for 10 min, discard the supernatant, then add 1 m L of 75% ethanol, mix well, and continue centrifugation at 4 °C and 10,000 g for 10 min; then air dry at room temperature, add 30 μL of DEPC-treated dd H2O water to dissolve the RNA, and store at −80 °C. Storage.
3
Synthesis of Functionalized Organic Compounds
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Citric
acid, maleicanhydride, toluene, 1,4-dioxane, p-toluenesulfonic
acid, epichlorohydrin, ethyl acetate (EtOAc),
NaOH, Na2SO4, 2,4-toluene diisocyanate (TDI),
glycidyl, chloroform, hydrogen peroxide (H2O2), and N,N-dimethylformamide (DMF)
were commercially supplied by Shanghai Chemical Reagent Co., Ltd.
All reagents were of analytical grade.
acid, maleicanhydride, toluene, 1,4-dioxane, p-toluenesulfonic
acid, epichlorohydrin, ethyl acetate (EtOAc),
NaOH, Na2SO4, 2,4-toluene diisocyanate (TDI),
glycidyl, chloroform, hydrogen peroxide (H2O2), and N,N-dimethylformamide (DMF)
were commercially supplied by Shanghai Chemical Reagent Co., Ltd.
All reagents were of analytical grade.
4
Ginkgo Fatty Acid Profiling
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All fatty acids (C5–C30) standards were of analytical grade and purchased from Sigma-Aldrich (St. Louis, MO, USA). HPLC grade acetonitrile (ACN) was purchased from Yucheng (Shandong Province, China). N,N-Dimethylformamide (DMF), potassium carbonate (K2CO3), pyridine, and chloroform were of analytical grade obtained from Shanghai Chemical Reagent Co. (Shanghai, China). Toluene-4-sulfonic acid 2-(2-thiophen-2-yl-phenanthro[9,10-d]imidazol-1-yl)-ethyl ester (TSTPE) was synthesized in our lab as described in the synthesis section and Fig. 1 . Water was purified on a Milli-Q system (Millipore, Bedford, MA). All other reagents used in this study were also of analytical grade unless otherwise stated. Ginkgo nut and ginkgo leaf samples were collected from Shandong province, Jiangsu province and Henan province of China.
5
Synthesis and Analysis of AEAO
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Standards of VB3 and VB7 were of chromatographic grade and purchased from the National Institute for Control of Pharmaceutical and Biological Products (Beijing, China). N-ethyl-N'-[(3-dimethylamino) propyl]carbondiiminehydrochloride (EDC), Pyridine and chloroform were of analytical grade and purchased from Shanghai Chemical Reagent Co. (Shanghai, China). HPLC-grade acetonitrile (ACN) was obtained from Yuwang Company, China. Water was purified on a Milli-Q system (Millipore, Bedford, MA, USA). All other reagents used were also of analytical grade unless otherwise stated. AEAO was synthesized in authors’ laboratory as described in section 2.3 and Fig 2 . Processed tea samples were bought from Hangzhou West Lake Longjing Industry Co.(June, 2011), Ltd. and Guizhou Fanjin Industry Co., Ltd.(July, 2011).
6
Thiol-Mediated Photoredox Polymerization for Advanced Materials
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1-Butanethiol (Energy Chemical, Shanghai, China, 98%), 2-bromopropionic acid (Energy chemical, Shanghai, China, 99%), tetrabutylammonium hydrogen sulfate (Aladdin, Shanghai, China, 99%), 2,2’-(thiocarbonyldithio)diacetic acid, N,N-dimethyl acrylamide (DMA) (Aldrich, St. Louis, MO, USA, 99%), N,N-dimethylaminoethyl acrylate (DMAEA) (Energy chemical, Shanghai, China, 98%), N,N-Diethylacrylamide (DEA) (Aladdin, Shanghai, China, 99%), N-(3-(dimethylamino)propyl)acrylamide (DPAA) (Energy Chemical, Shanghai, China, 98%), 3-mercaptopropionic acid (Aladdin, Shanghai, China, 99%), 2-Bromoisobutyric acid (Energy chemical, Shanghai, China, 98%), 4,7-Di(thiophen-2-yl)benzo[c][1,2,5]thiadiazole (DTBT as photoredox catalyst in this study) (ARK, Chicago, IL, USA, >98.0%), bis(carboxymethyl) trithiocarbonate, CAS: 6326-83-6) (BCMT) (Energy Chemical, Shanghai, China, 98%), N, N-dimethylformamide (DMF) (Aladdin, Shanghai, China, 99.8%) were purchased from chemical company, respectively. Acetone, hydrochloric acid, carbon disulfide, chloroform, DCM, hexane, ether, K3PO4·3H2O, NaCl, NaOH, D2O, CDCl3, anhydrous Na2SO4 were obtained from Shanghai Chemical Reagent Co., Shanghai, China.
7
Fluoxetine Effects on ERK1/2 and BDNF
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20 mg/granules fluoxetine hydrochloride capsules were purchased from Lilly Suzhou Pharmaceutical Co., Ltd. Rabbit anti-mouse ERK1/2 antibody was purchased from the American Abcam company. Rabbit anti-mouse BDNF antibody was purchased from Abcam, USA. The PCR reverse transcription kit was purchased from Promega. Rabbit anti-mouse pERK1/2 antibody was purchased from Abcam, USA. The normal goat serum for closure was purchased from the American Abcam company. The streptavidin-biotin-horseradish peroxidase complex was purchased from VECTOR Bio GmbH, USA. The SABC kit was purchased from VECTOR Bio GmbH, USA. The BCA protein quantification kit was purchased from the American Abcam company. TRIzol was purchased from the Invitrogen company. Chloroform, isopropanol, and absolute ethanol were of analytical grade and were purchased from Shanghai Chemical Reagent Company. DNA marker was purchased from Beijing Dingguo Biotechnology Company.
8
Moeixitecan: A Novel Nanomedicine
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2-(hexadecyloxycarbonyl)-2, 5, 7, 8-tetramethylchroman-6-yl 7-ethyl-camptothecin-10-yl succinate (moeixitecan) obtained internally by Chia-tai Tianqing Pharmaceutical (Nanjing, People's Republic of China). 7-Ethyl-10-hydroxycamptothecin (SN38) was provided by Sigma-Aldrich (Shanghai). Dipalmitoyl phosphatidylcholine (DPPC), hydrogenated soybean phospholipids (HSPC), and distearoyl phosphoethanolamine-PEG2000 (DSPE-PEG2000) were purchased from Southeast Pharmaceuticals Co. (Soochow, People's Republic of China). Chloroform and methanol were purchased from Shanghai Chemical Reagent Company.
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