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Mouse igg total elisa kit

Manufactured by Thermo Fisher Scientific
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The Mouse IgG Total ELISA kit is a quantitative enzyme-linked immunosorbent assay (ELISA) designed for the measurement of total mouse immunoglobulin G (IgG) levels in various sample types, such as serum, plasma, or cell culture supernatants.

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Lab products found in correlation

Nunc maxisorp plate, by Thermo Fisher Scientific (1 mentions) Mouse ana total ig elisa kit, by Alpha Diagnostic (1 mentions) Anti ovalbumin igg1 mouse elisa kit, by Cayman Chemical (1 mentions) Spin x tube, by Corning (1 mentions) Aluminum hydroxide, by Merck Group (1 mentions) Poloxamer 407, by Merck Group (1 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions)

5 protocols using mouse igg total elisa kit

1

Quantifying Mouse Serum IgG and ANA

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Mouse serum or plasma was assessed via the IgG (Total) Mouse ELISA Kit (Thermo Fisher), according to the manufacturer’s directions, using 96-well Nunc Maxisorp plates (Thermo Fisher) with samples diluted 1:20,000 – 1:40,000. Serum or plasma was assessed for ANA using the Mouse ANA Total Ig ELISA Kit (Alpha Diagnostic International), according to the manufacturer’s directions, with samples diluted 1:50. Total IgG and ANA ELISA plates were read using a GlowMax Discover Microplate Reader (Promega) on the absorbance setting at 450nm.
Reighard S.D., Krishnamurthy D., Cevik H., Ochayon D.E., Ali A., Seelamneni H., Brunner H.I, & Waggoner S.N. (2020). Immunomodulatory effects of cytokine-induced expansion of cytotoxic lymphocytes in a mouse model of lupus-like disease. Cytotherapy, 23(1), 37-45.
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2

IgG1 Quantification from Mouse Serum

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Blood was collected by cardiac puncture at endpoints and serum isolated by coagulation and centrifugation. Serum aliquots were snap-frozen at −80° C until use. Total IgG1 was measured using IgG (Total) Mouse ELISA Kit (Thermo) and OVA-specific IgG1 was measured using Anti-Ovalbumin IgG1 (mouse) ELISA Kit (Cayman Chemical) on duplicate sera samples diluted 1:10,000 and 1:100 respectively. Absorbance values within dynamic range were read at 450 nm and 570 nm, and final concentrations were interpolated on Sigmoidal (4PL) fit of standard-curve.
Hegde S., Krisnawan V.E., Herzog B.H., Zuo C., Breden M.A., Knolhoff B.L., Hogg G.D., Tang J.P., Baer J.M., Mpoy C., Lee K.B., Alexander K.A., Rogers B.E., Murphy K.M., Hawkins W.G., Fields R.C., DeSelm C.J., Schwarz J.K, & DeNardo D.G. (2020). Dendritic cell paucity leads to dysfunctional immune surveillance in pancreatic cancer. Cancer cell, 37(3), 289-307.e9.
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3

Serum Transfer for Immunomodulation in Mice

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Blood was collected from normal C57BL/6 mice and allowed to clot in a 1.7 ml tube and then serum was separated by centrifugation at 8000 rpm for 10 minutes. Serum was stored at −80°C after filtration in 0.22 µm Spin-X tube (Costar). Control or µMT mice were injected i.v. with 500 µL serum one day before dead cells were injected or acetaminophen treatment. To confirm the efficiency of serum transfer, at the time of peritoneal lavage or liver harvest serum was taken from each mouse and total IgG was measured by Mouse IgG Total ELISA kit according to the manufacturer's protocol (eBioscience).
Kataoka H., Kono H., Patel Z, & Rock K.L. (2014). Evaluation of the Contribution of Multiple DAMPs and DAMP Receptors in Cell Death-Induced Sterile Inflammatory Responses. PLoS ONE, 9(8), e104741.
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4

Polymeric Nanoparticle Formulation Protocol

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D,L-PLGA 50:50 (lactide:glycolide) (inherent viscosity 0.63 dL·g−1 at 30 °C) was purchased from Birmingham Polymers Inc. (Alabaster, AL, USA); poloxamer 407, polyethyleneimine (PEI) branched average molecular weight 25,000 Da and aluminum hydroxide were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). BCA Protein Assay Kit was purchased from Pierce Biotechnology (Woburn, MA, USA), and Mouse IgG total ELISA Kit from eBioscience (San Diego, CA, USA). The purified water (1.3 µS·cm−1) was prepared from reverse osmosis purification equipment (model OS50 LX, Gehaka, São Paulo, Brazil). All other reagents were of analytical grade.
dos Santos-Silva E., Torres-Rêgo M., Gláucia-Silva F., Feitosa R.C., Lacerda A.F., Rocha H.A., Fernandes-Pedrosa M.D, & da Silva-Júnior A.A. (2022). Cationic PLGA Nanoparticle Formulations as Biocompatible Immunoadjuvant for Serum Production and Immune Response against Bothrops jararaca Venom. Toxins, 14(12), 888.
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5

Measurement of Anti-MOG Antibodies

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Mouse IgG total was measured in plasma with the mouse IgG total ELISA kit (eBioscience). Levels of anti-MOG Abs in mouse plasma were performed using indirect ELISAs. In brief, ELISA plates were coated with 10 μg/mL MOG35–55 peptide. Plates were probed with 1/400 dilutions of serum from individual mice, and reactive Abs were detected using peroxidase-conjugated goat and anti-mouse specific for IgG (Southern Biotech, Birmingham, AL) and developed with tetramethylbenzidine.
Kumar G., Maria Z., Kohli U., Agasing A., Quinn J.L., Ko R.M., Zamvil S.S, & Axtell R.C. (2021). CNS Autoimmune Responses in BCMA-Deficient Mice Provide Insight for the Failure of Atacicept in MS. Neurology® Neuroimmunology & Neuroinflammation, 8(3), e973.
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