Lgr5 egfp

Manufactured by Jackson ImmunoResearch

Lgr5-EGFP is a reporter system that incorporates the Lgr5 gene, which is a marker for intestinal stem cells, fused with the EGFP (enhanced green fluorescent protein) gene. This system allows for the visualization and identification of Lgr5-positive cells, which are crucial for the study of intestinal stem cell biology.

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Lab products found in correlation

Lgr5 egfp ires creert2 mice, by Jackson ImmunoResearch (1 mentions) Wild type c57bl 6j mice, by Jackson ImmunoResearch (1 mentions) Villin cre, by Jackson ImmunoResearch (1 mentions) Apcmin, by Jackson ImmunoResearch (1 mentions) Cmv cre, by Jackson ImmunoResearch (1 mentions) Nod scid, by Jackson ImmunoResearch (1 mentions) Actin egfp, by Jackson ImmunoResearch (1 mentions) Wild type c57bl 6 mice, by Jackson ImmunoResearch (1 mentions)

4 protocols using lgr5 egfp

Generating Transgenic Lgr5 Mice to Study Intestinal Stem Cells

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C57BL/6J wild type (WT) mice and B6129P2-Lgr5^{tm1(cre/ERT)Cle}/J mice with Lgr5-EGFP expression confined to ISC (hereafter referred to as Lgr5 TG mice) were purchased from the Jackson Laboratory (Bar Harbor, ME). To generate B6129P2-Lgr5^{tm1(cre/ERT)Cle}/J;Villi-HB-EGF/+ mice (Lgr5 TG mice that overexpress HB-EGF in the intestines; hereafter referred to as Lgr5-HB-EGF TG mice), B6129P2-Lgr5^{tm1(cre/ERT)Cle}/J mice were crossed with homogeneous transgenic Villi-HB-EGF/Villi-HB-EGF mice that we produced in our laboratory.²⁷ In addition, Lgr5-lacZ mice were generated by crossing Lgr5-EGFP-IREScreERT2 mice to Rosa26-lacZ Cre-reporter mice, both obtained from Jackson Laboratory (Bar Harbor, ME).^{20 (link)} One to two month old mice were injected intraperitoneally (IP) with tamoxifen (200 μl;10 mg/ml) in sunflower oil to induce LacZ expression in Lgr5⁺ ISC and their progeny cells.

Chen C.L., Yang J., James I.O., Zhang H.Y, & Besner G.E. (2014). Heparin-binding EGF-like Growth Factor Restores Wnt/β-catenin Signaling in Intestinal Stem Cells Exposed to Ischemia/Reperfusion Injury. Surgery, 155(6), 1069-1080.

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Lgr5-EGFP Mouse Model for Stem Cell Research

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Lgr5-EGFP-Ires-CreERT2 (Lgr5-EGFP) mice (Barker et al., 2007 (link)) (Jackson Laboratory, Stock No. 00887) and FVB mice used as wide-type mice were raised in a comfortable environment with suitable temperature and light and fed with standard laboratory food and water ad libitum. We are approved by the Animal Care and Use Committee of Southeast University and were consistent with the National Institutes of Health Guide for the Care and Use of Laboratory Animals. All the operations were carried out in accordance with the procedures.

Chen Y., Qiang R., Zhang Y., Cao W., Wu L., Jiang P., Ai J., Ma X., Dong Y., Gao X., Li H., Lu L., Zhang S, & Chai R. (2021). The Expression and Roles of the Super Elongation Complex in Mouse Cochlear Lgr5+ Progenitor Cells. Frontiers in Cellular Neuroscience, 15, 735723.

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Transgenic Mouse Strains for Intestinal Research

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CMV-Cre (Schwenk et al. 1995 (link)), Apc^min/+ (Moser et al. 1990 (link)), Villin-Cre (Madison et al. 2002 (link)), and Lgr5^+/eGFP (Barker et al. 2007 (link)) mice were obtained from Jackson Laboratory. Strains used in this study were backcrossed to C57BLJ/6 mice for at least 10 generations. Dox (2 mg/mL, supplemented with 10 mg/mL sucrose) was administered in the drinking water to induce transgene expression. All experiments were approved by the Institutional Animal Care and Use Committees of the Johns Hopkins University School of Medicine and The University of Texas Southwestern Medical Center.

Zeitels L.R., Acharya A., Shi G., Chivukula D., Chivukula R.R., Anandam J.L., Abdelnaby A.A., Balch G.C., Mansour J.C., Yopp A.C., Richardson J.A, & Mendell J.T. (2014). Tumor suppression by miR-26 overrides potential oncogenic activity in intestinal tumorigenesis. Genes & Development, 28(23), 2585-2590.

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Mouse Models in Biomedical Research

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All experiments involving mice were carried out according to the protocols approved by the IACUC of Children's Hospital Los Angeles, which adheres to the US National Institute of Health's Office of Laboratory Animal Welfare Guidelines (Approval #215). C57BL/6 wild type mice (000664—The Jackson Laboratory), Actin^egfp (Sala et al., 2011 (link)), Lgr5^egfp (008875—The Jackson Laboratory), and non-obese diabetic/severe combined immunodeficient mice (NOD/SCID, 005557—The Jackson Laboratory) were maintained on C54BL/6 background.

Levin G., Zuber S.M., Squillaro A.I., Sogayar M.C., Grikscheit T.C, & Carreira A.C. (2020). R-Spondin 1 (RSPO1) Increases Mouse Intestinal Organoid Unit Size and Survival in vitro and Improves Tissue-Engineered Small Intestine Formation in vivo. Frontiers in Bioengineering and Biotechnology, 8, 476.

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