Ddr2 400

The “grafting through” method was used to produce polymers with a brush structure. The synthesis of the polymer brushes by the “grafting through” method implies a one-step process using macromonomers capable of radical polymerization. Three macromonomers with different oligo(ethylene glycol) (OEG) and oligo(propylene glycol) (OPG) block lengths were used to obtain polymers (Dzerzhinsk Polytechnic Institute, Dzerzhinsk, Russia; Sigma-Aldrich, Saint Louis, MO, USA). We used OEGeOPGpMA with the following average lengths of oligo(ethylene glycol) (e) and oligo(propylene glycol) (p) fragments: e = 7.0 and p = 2.8 in copolymer E7P3, e = 7.0 and p = 5.4 in brush E7P5, and e = 7.0 and p = 10.3 in sample E7P10 (Dzerzhinsk Polytechnic Institut, Dzerzhinsk, Russia). Polymers were synthesized by the conventional free-radical polymerization in organic solvents at 60–85 °C. The synthesis procedure of OEGeOPGpMAs was described in detail previously [34 ].
The structure of the polyOEGeOPGpMA samples was confirmed by nuclear magnetic resonance (NMR) spectroscopy (DDR2 400; Agilent, Santa Clara, CA, USA) in DMSO-D6 (Figure S1 in Supplementary Materials).

The first sample of poly(N-[3-(diethylamino)propyl]methacrylamide) (PDEAPMA-C) was synthesized by free radical polymerization. The PDEAPMA-C was obtained in glass ampoules in toluene solution at a temperature of 60 °C for 4 h. The reaction mixture was purged with nitrogen before polymerization. The concentration of DEAPMA was 20 wt.%. Concentration of the initiator (azobisisobutyronitrile) was 1 mol. % from the amount of the monomer. The polymer obtained was isolated by precipitation in cold hexane and dried in a vacuum. The IR and ¹H NMR (DDR2 400; Agilent, Santa Clara, CA, USA) spectrum of the polymer are given in Supplementary Materials (Figure S1).

¹H NMR and ¹³C NMR spectra were recorded in DMSO-d₆ at 25 °C on an Agilent DDR2 400 spectrometer with operating frequencies of 400 MHz for ¹H and 101 MHz for ¹³C. Chemical shifts (δ) are reported in parts per million (ppm) from TMS using the residual solvent resonance (DMSO-d₆: 2.50 ppm for ¹H NMR, 39.52 ppm for ¹³C NMR). Signal assignments in the proton spectra were based on comparison [13 (link),14 (link)]. Mass spectra were recorded on a DSQ mass spectrometer with a quadrupole mass analyzer. The temperature of the ion source was 230 °C and ionization was carried out by electrons with an energy of 70 eV. Elemental analyses were performed on an Elementar (Vario Micro Cube) instrument. Column chromatography was performed using Merck Kieselgel 60 (70–230 mesh). Commercially available reagents (Aldrich, AlfaAesar and Acros) were used without additional purification. Solvents were purified according to the standard procedures. The petroleum ether (PE) used corresponds to the 40–70 °C fraction.