In our institution, all patients with PSA 4 ng/mL or higher did undergo biopsy as the standard of care. All biopsy-naïve patients in this study underwent a 10-core systematic transrectal ultrasound- (TRUS-) guided prostate biopsy. The 10-core biopsies were obtained from the base (2 cores), midgland (2 cores), and apex (1 core) from each side of the prostate. Patients with negative MRI (PI-RADS v2.1 scores 1 and 2) underwent a standard 10-core systematic TRUS-guided prostate biopsy only. For suspicious PCa lesions on MRI (PI-RADS v2.1 score ≥ 3), an MRI-TRUS fusion-guided targeted biopsy was used, and then, 2-3 targeted cores would be added for these lesions. The MRI-TRUS fusion-guided targeted biopsy was performed using Esaote's MyLab Twice ultrasound system (Esaote, Italy). Histopathologic evaluation of the biopsy specimens was analyzed and reported by experienced genitourinary pathologists in our institution according to the International Society of Urological Pathology (ISUP) 2014 updated Gleason score grading system [24 (link), 25 (link)]. A Gleason score ≥ 7 on the MRI-TRUS fusion targeted biopsy and/or a matching segment on a systematic TRUS-guided prostate biopsy was considered positive for cs-PCa.
Mylab twice ultrasound system
Manufactured by Esaote
Sourced in Italy
The MyLab Twice ultrasound system is a medical imaging device developed by Esaote. It is designed to provide high-quality ultrasound imaging for diagnostic purposes. The core function of the MyLab Twice is to generate and process ultrasound waves to create visual representations of the internal structures of the human body.
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Lab products found in correlation
6 protocols using mylab twice ultrasound system
1
Multiparametric MRI-Guided Prostate Biopsy Protocol
2
Multimodal Imaging of IR783-NBs-Affibody
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Optical imaging capability of IR783-NBs-Affibody was observed by laser scanning confocal microscopy (LSCM, Nikon A1R, Japan) with excitation at 640 nm and peak emission at 780 nm to obtain NIR fluorescent.
To complete ultrasound contrast imaging, a latex glove fingertip filling with 10 mL of 1x PBS was put in water at 25°C. MyLab Twice ultrasound system (Esaote, Italy) was used to acquire ultrasound images with a 5 MHz transducer. After the ultrasound images of PBS-containing glove can be acquired clearly, SonoVue and IR783-NBs-Affibody (100 μL) were injected into PBS, respectively, then the ultrasound pictures were compared.
To complete ultrasound contrast imaging, a latex glove fingertip filling with 10 mL of 1x PBS was put in water at 25°C. MyLab Twice ultrasound system (Esaote, Italy) was used to acquire ultrasound images with a 5 MHz transducer. After the ultrasound images of PBS-containing glove can be acquired clearly, SonoVue and IR783-NBs-Affibody (100 μL) were injected into PBS, respectively, then the ultrasound pictures were compared.
3
Ultrasound-Triggered Nds-IR780 Phase Change
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First, a latex glove fingertip containing 10 mL degassed water was immersed in a water bath with an ultrasound transducer on one side. Then, 2 mL of the above Nds-IR780 (20×) solution was injected into the fingertip, and ultrasound contrast imaging was recorded by a Mylab Twice Ultrasound System (Esaote, Italy) in visualization mode with a 7.5 MHz transducer. Afterwards, the fingertip containing Nds-IR780 was irradiated by low frequency ultrasound (20 s, 2.5 w/cm2, continuous irradiation). Then, the ultrasound contrast imaging was again examined, and the results were saved on the computer. Both before and after the phase change of Nds-IR780, a drop of solution was taken and set on a glass slide, and the morphological change of Nds-IR780 was observed through optical microscopy.
4
Contrast-Enhanced Ultrasound Imaging for Tumor Detection
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First, the tumor-bearing nude mouse was anesthetized through intraperitoneal injection using 100 μL of 1% sodium pentobarbital and placed on a plate for imaging. Then, the ultrasound probe was placed on the tumor and groin using the 2-D and color Doppler imaging modes. Next, 200 μL of the Nds-IR780 solution (2.4×107 nanodroplets/mL) was injected into the mouse via the tail vein. After 1 h, the tumor was irradiated by placing the low frequency ultrasound transducer (20 s, 2.5 w/cm2, continuous irradiation) on the tumor surface; contrast-enhanced ultrasound imaging was then performed with a Mylab Twice Ultrasound System (Esaote, Italy) with a 7.5 MHz transducer. The above data and images were stored for offline examination. After 8 h, the tumor-bearing mouse was anesthetized using isoflurane and NIRF imaging was performed via an IVIS Lumina II imaging station (Caliper Life Sciences, Hopkinton, MA, USA). All of the above experiments were repeated at least three times.
5
Breast Ultrasound Imaging Protocol
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US images were obtained using a 5–12 MHz linear probe and a VISION Ascendus ultrasound system (Hitachi Aloka Medical, Tokyo, Japan), a Voluson E8 Exp (GE Medical Systems, Zipf, Austria), an Apolio 500 platform (Toshiba, Otawara, Japan), a MyLab Twice ultrasound system (Esaote, Genoa, Italia), an iU22 scanner (Philips Ultrasound, Bothell, WA, USA), and an Aixplorer ultrasound system (SuperSonic Imagine, Aix en Provence, France).
Two independent radiologists with more than 10 years’ working experience in breast US retrospectively evaluated the sonography features of the lesions using the American College of Radiology breast imaging reporting and data system (ACR-BIRADS) 5th edition. Nodule features including location, multiplicity, size, shape, margin, orientation, echogenicity, calcification, blood flow signal of the lesions, and axillary LN status were recorded. In addition, associated features, including skin thickening, edema, and architectural distortion were also evaluated by ultrasonography. If two radiologists had different opinions, all disagreements were discussed to the point of consensus. If the patients presented with multiple breast nodules, the largest was evaluated.
Two independent radiologists with more than 10 years’ working experience in breast US retrospectively evaluated the sonography features of the lesions using the American College of Radiology breast imaging reporting and data system (ACR-BIRADS) 5th edition. Nodule features including location, multiplicity, size, shape, margin, orientation, echogenicity, calcification, blood flow signal of the lesions, and axillary LN status were recorded. In addition, associated features, including skin thickening, edema, and architectural distortion were also evaluated by ultrasonography. If two radiologists had different opinions, all disagreements were discussed to the point of consensus. If the patients presented with multiple breast nodules, the largest was evaluated.
6
Measurement of IR780-NBs-DTX CEUI in vitro
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The CEUI capability of IR780-NBs-DTX in vitro was measured by an analytical setup (Fig. 2 a). A latex glove fingertip containing 10 mL of degassed water was set in a degassed water bath with an ultrasound transducer on one side. Two hundred microliters of different concentrations of IR780-NBs-DTX suspensions (with an initial addition of 0.15 mg IR-780 iodide/1.0 mg DTX; the parent suspension was diluted 5 times: 1.2 × 107 bubbles/mL, 10 times: 6 × 106 bubbles/mL, 20 times: 3 × 106 bubbles/mL) were injected into the latex glove fingertip in turn, and SonoVue and PBS were used as controls. The CEUI was recorded by a Mylab Twice Ultrasound System (Esaote, Italy) with a 7.5 MHz transducer.The concentration of the bubbles was controlled via a hemocytometer, and the number of bubbles counted was the same as the number of cells, and the concentration was calculated using the same cell-counting method.
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