Agilent 6230 lc msd tof system

All capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) experiments were performed using Agilent 7100 CE capillary electrophoresis (Agilent Technologies, Waldbronn, Germany), the Agilent 6230 LC/MSD TOF system (Agilent Technologies, Palo Alto, CA, USA), an Agilent1100 series binary HPLC pump, and the G1603A Agilent CE-MS adapter- and G1607A Agilent CE-ESI–MS sprayer kit. For anionic metabolite analysis, the original Agilent stainless electrospray ionization (ESI) needle was replaced with an Agilent G7100-60,041 platinum ESI needle (Soga et al., 2009 (link)). System control and data acquisition were performed using the Agilent MassHunter Workstation, and data analysis was performed using Keio MasterHands software.

All capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) experiments were performed using Agilent 7100 CE capillary electrophoresis (Agilent Technologies), the Agilent 6230 LC/MSD TOF system (Agilent Technologies), an Agilent1100 series binary HPLC pump, and the G1603A Agilent CE-MS adapter- and G1607A Agilent CE-ESI-MS sprayer kit. For anionic metabolite analysis, the original Agilent stainless ESI needle was replaced with the Agilent G7100-60041 platinum ESI needle.⁶⁸ (link) System control and data acquisition were performed by Agilent MassHunter Workstation, and data analysis was done by Keio MasterHands software.