Agmatine, LPS (Escherichia coli 0111:B4) and protoporphyrinIX zinc(II) (ZnPP)were purchased from Sigma–Aldrich (Saint Louis, MO, USA). LY294002, protease/phosphatase inhibitor cocktail, anti-β-actin, anti-phosphorylated Akt and anti-total Akt antibodies were products of Cell Signaling Technology (Danvers, MA, USA). Idazoxan, anti-Nrf2 and anti-iNOS antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).Anti-HO-1 antibody was product of Abcam (Cambridge, MA, USA). RPMI 1640 and fetal bovine serum were products of Gibco-BRL Invitrogen (San Diego, CA, USA). Yohimbine, efaroxan, dimethyl sulfoxide (DMSO), 2′,7′-dichlorofluorescin diacetate (DCFH-DA) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were obtained from Sigma-Aldrich. BCA protein assay kit, total nitric oxide assay kit and nuclear and cytoplasmic protein extraction kits were from Beyotime (Jiangsu, China).
Idazoxan
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Idazoxan is a selective alpha-2 adrenergic receptor antagonist. It is commonly used in research applications to investigate the role of alpha-2 adrenergic receptors in various physiological and pharmacological processes.
Automatically generated - may contain errors
Lab products found in correlation
Efaroxan, by Merck Group (2 mentions)
Total nitric oxide assay kit, by Beyotime (1 mentions)
Agmatine, by Merck Group (1 mentions)
Anti inos, by Santa Cruz Biotechnology (1 mentions)
Anti nrf2, by Santa Cruz Biotechnology (1 mentions)
Anti ho 1 antibody, by Abcam (1 mentions)
Nuclear and cytoplasmic protein extraction kit, by Beyotime (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
2 7 dichlorofluorescin diacetate dcfh da, by Merck Group (1 mentions)
Protoporphyrin 9 zinc 2 znpp, by Merck Group (1 mentions)
Anti total akt, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Anti phosphorylated akt, by Cell Signaling Technology (1 mentions)
Yohimbine, by Merck Group (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Lps escherichia coli 0111 b4, by Merck Group (1 mentions)
Protease phosphatase inhibitor cocktail, by Cell Signaling Technology (1 mentions)
2 protocols using idazoxan
1
Agmatine Modulation of Inflammation
2
Platelet Activation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dexmedetomidine and idazoxan were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Yohimbine and methylene blue were purchased from Nacalai Tesque (Kyoto, Japan). Levomedetomidine, an S (-)-enantiomer of medetomidine was kindly supplied by Orion Corporation (Espoo, Finland). Adenosine diphosphate (ADP) and efaroxan were purchased from Sigma-Aldrich (St. Louis, MO, USA), and Tocris Bioscience (Bristol, UK), respectively. 9, 11epithio-11, 12-methanothromboxane A 2 (STA 2 ) was provided by Ono Pharmaceutical (Osaka, Japan). Peridinin Chlorophyll Protein (PerCP)-labeled anti-CD61 antibody, Phycoerythrin (PE)labeled anti-CD62P (P-selectin) antibody and PE-labeled IgG for control were obtained from Becton Dickinson (San Diego, CA, USA). All other chemicals were of analytical grade. We confirmed that all buffers or solvents for diluting materials in our experiments showed no effect on the results.
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