Streptavidin 594

Manufactured by Thermo Fisher Scientific

Sourced in Japan

Streptavidin-594 is a fluorescently labeled protein that binds to biotin with high affinity. It can be used in various biomedical research applications that utilize the biotin-streptavidin interaction.

Automatically generated - may contain errors

Lab products found in correlation

Biotinylated secondary antibody, by Vector Laboratories (1 mentions) Mms 435p, by Merck Group (1 mentions) Sc 7819, by Santa Cruz Biotechnology (1 mentions) Alexa488 and alexa594 conjugated secondary antibodies, by Thermo Fisher Scientific (1 mentions) Biotinylated anti goat, by Vector Laboratories (1 mentions) Phosphate buffered saline (pbs), by BioLegend (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Draq5, by Cell Signaling Technology (1 mentions) Phalloidin, by Thermo Fisher Scientific (1 mentions) Slide chambered coverslips, by Ibidi (1 mentions) Hoechst, by Thermo Fisher Scientific (1 mentions)

3 protocols using streptavidin 594

Immunohistochemistry for Neuronal Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

We used the following antibodies: ChAT (Chemicon AB144P), Otx2 (R&D systems goat, catalog # AF1979), Tuj1 (Covance MMS-435P), pH3 (Millipore 06-570), parvalbumin (Millipore MAB1572), somatostatin (Santa Cruz sc-7819).
Cryosections were rinsed in PBS, blocked in 10% normal serum/PBST (1x PBS, 0.1% Triton X-100), incubated in primary antibody overnight (4° C), washed in PBST, incubated in secondary antibody 1–3 hours (room temperature), and washed in PBS. For fluorescent detection, we used Alexa 488- and Alexa 594-conjugated secondary antibodies (Invitrogen). For colorimetric detection, biotinylated secondary antibodies (Vector) were used with the ABC (Vector)/DAB detection method.
For ChAT IHC, antigen retrieval was achieved by incubating slides in 2.94g/L trisodium citrate dehydrate, 0.05% Tween-20, pH 6.0 for 15 minutes at 90° C. Blocking and antibody incubations were done in 1% BSA in PBST. Sections were incubated two days at 4° C with primary antibody, and signal was amplified with biotinylated anti-goat (Vector) prior to fluorescent detection with streptavidin-594 (Invitrogen).
For OTX2 IHC, we modified the IHC protocol according to the recommendations of Yuki Muranishi in the Furakawa lab (Osaka, Japan). Briefly, antigen retrieval was achieved as for ChAT IHC, and samples were blocked in 4% donkey serum in PBST.

Hoch R.V., Lindtner S., Price J.D, & Rubenstein J.L. (2015). OTX2 Transcription Factor Controls Regional Patterning Within The Medial Ganglionic Eminence (MGE) And Regional Identity Of The Septum. Cell reports, 12(3), 482-494.

+ Open protocol

+ Expand

Visualizing CXCL12 Binding in Embryonic Hearts

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hearts from wild-type E11.5 or E12.5 embryos were dissected into PBS (GIBCO, 14190-094). Following a brief incubation in PBS + 2% FBS at 37°C, tissue was blocked in DMEM containing 3.78uM unlabelled streptavidin (BioLegend, 405150) for 30 minutes at 37°C. Samples were washed twice in PBS + 2% FBS, and then incubated in media containing 200nM biotinylated-CXCL12 (Generon, OPCB00014) conjugated to streptavidin-594 (Invitrogen, S11227) for 30 minutes at 37°C. The biotinylated-CXCL12: streptavidin-594 conjugate was formed by incubating these components in a 1:4 molar ratio at room temperature for 3 minutes immediately prior to addition to the tissue, as per manufacturer’s instructions. For competition experiments, samples were co-incubated with 2μM unlabelled CXCL12 (R&D Systems, 350-NS). Samples were washed twice in ice cold DMEM, blocked with PBS + 0.5% BSA for 5 minutes at 4°C, fixed with 4% PFA for 10 minutes, and then washed twice with PBS at 4°C. Specimens were then embedded in OCT for cryosectioning and immunohistochemistry with anti-PECAM-1 antibody as described in ‘General Immunohistochemistry and Quantification’ with the exception that blocking was performed using PBS + 2%BSA. Images were acquired on a spinning disk microscope, as detailed above. Images were exported to FIJI and processed for publication.

Ridge L.A., Kewbank D., Schütz D., Stumm R., Scambler P.J, & Ivins S. (2021). Dual role for CXCL12 signaling in semilunar valve development. Cell Reports, 36(8), 109610.

+ Open protocol

+ Expand

Immunofluorescent Labeling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunofluorescent labelling, cells were seeded into 8 or 4 well µ-Slide chambered coverslips (Ibidi). Following the experimental procedures, cells were fixed using 4% PFA and quenched in 50 mM NH4Cl. Cells were permeabilized with 0.5% Saponin in 2% BSA-PBS then antibodies were diluted in the same buffer and incubated with the cells overnight at 4°C. Following three washing steps with PBS, secondary antibodies were applied for 1 h together with Hoechst (Invitrogen) and other dyes such as DraQ5 (Cell Signaling Technology), Phalloidin (Invitrogen) and Streptavidin594 (Invitrogen) depending on the experiment.

Kovács D., Gay A., Fleuriot L., Debayle D., Araújo A.R.D., Patel A., Mesmin B., Luton F., & Antonny B. (2021). OSBP-mediated cholesterol transfer determines epithelial polarity and associated cargo secretion.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!