Hepatocytes were seeded at a density of 2 × 104 cells per well in a six-well plate (Thermo Scientific™, Nunc™; Waltham, MA, USA) and maintained as described above for 2 weeks with daily changes in medium. Viable cells were monitored and counted daily using a hemocytometer and an Olympus IX71 inverted phase/fluorescence microscope (Olympus, Tokyo, Japan). Growth curves were generated using GraphPad Prism software, version 7.0 (GraphPad Inc, La Jolla, CA, USA).
Ix71 inverted phase fluorescence microscope
Manufactured by Olympus
Sourced in Japan
The IX71 is an inverted phase/fluorescence microscope manufactured by Olympus. It is designed for a wide range of applications, including cell culture observation, live-cell imaging, and fluorescence imaging. The IX71 features a stable optical system and a variety of illumination options to support various imaging techniques.
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Lab products found in correlation
2 protocols using ix71 inverted phase fluorescence microscope
1
Hepatocyte Seeding and Growth Monitoring
2
Neuroprotective Effects of YC-1 against Glutamate-Induced Toxicity
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Cells were administered the following experimental treatments: i) Pre-treatment group were administered YC-1 (1–100 µM) or vehicle (0.1% DMSO) for 30 min and then exposed to glutamate (300 mM) for 24 h; ii) post-treatment group in which YC-1 (30 µM) or vehicle (0.1% DMSO) was added to the culture medium at 0, 2, 4 or 6 h following glutamate exposure. The neuronal cytotoxicity was determined at 24 h following treatment using a PI assay kit (Thermo Fisher Scientific, Inc.). PI positive cell and total cell numbers were measured on an Olympus IX71 inverted phase/fluorescence microscope, equipped with a cooled charge-couple device camera as above. PI %=[(PI positive cell numbers)/(total cell numbers)] × 100.
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