Goat anti human fc specific antibody

Manufactured by Merck Group

Sourced in United States

Goat anti-human Fc-specific antibody is a laboratory reagent used in immunological assays and research applications. It is a polyclonal antibody produced in goats that specifically binds to the Fc region of human immunoglobulins.

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Lab products found in correlation

C digit blot scanner, by LI COR (4 mentions) Human serum, by Merck Group (3 mentions) Goat anti human igg h l antibodies, by Jackson ImmunoResearch (2 mentions) Nitrocellulose membrane, by Genesee Scientific (2 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Protein g sepharose, by GE Healthcare (2 mentions) Human male ab plasma, by Merck Group (1 mentions) Superdex 200 5 150 gl column, by GE Healthcare (1 mentions) Polyvinylidene difluoride membrane, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Lonza (1 mentions) Surface plasmon resonance, by Cytiva (1 mentions) Yarra 3 μm sec 3000 column, by Phenomenex (1 mentions) Hrp conjugated goat anti human igg h l antibody, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

Goat anti-human IgG (Fc specific)-Peroxidase antibody (3 citations) Goat anti-Human IgG (Fc specific)-FITC antibody (2 citations)

4 protocols using goat anti human fc specific antibody

Antibody Depletion and Detection in Human Serum

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Human serum (Sigma-Aldrich, St. Louis, MO, USA) was depleted of
endogenous IgG by passage through a protein G-Sepharose column. Antibodies and
ADCs were incubated at a concentration of 40 μg/ml in IgG-depleted serum
for 0, 3, or 5 days at 37 °C followed by immunoprecipitation using
agarose beads coupled to goat anti-human Fc-specific antibody (Sigma-Aldrich).
Immunoprecipitated antibodies and ADCs were run on SDS-polyacrylamide gels and
transferred onto nitrocellulose membranes (0.45 μm pore size; Genesee
Scientific, San Diego, CA, USA). Immunoblotting was carried out using standard
methods with goat anti-human IgG (H + L) antibodies conjugated with HRP (Jackson
Immunoresearch, West Grove, PA, USA). HRP was detected using SuperSignal West
Pico Chemiluminescent Substrate (Thermo Fisher Scientific) followed by scanning
with a C-DiGit blot scanner (LI-COR Biosciences, Lincoln, NE, USA).

Kang J.C., Sun W., Khare P., Karimi M., Wang X., Shen Y., Ober R.J, & Ward E.S. (2019). Engineering a HER2-specific antibody-drug conjugate to increase lysosomal delivery and therapeutic efficacy. Nature biotechnology, 37(5), 523-526.

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Antibody Depletion and Detection in Human Serum

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Serum Stability Evaluation of Seldegs

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For serum stability assays, endogenous IgGs were depleted from human male AB plasma (Sigma) by passage through protein G-Sepharose (GE Healthcare). Seldegs were incubated in serum at a concentration of 100 nM at 37 °C for 3 or 5 days. Following incubation, Seldegs were immunoprecipitated using agarose beads coupled to goat anti-human Fc-specific antibody (Sigma). Immunoprecipitated Seldegs were run on 12% SDS–polyacrylamide gels, transferred to polyvinylidene difluoride membranes (Millipore) and membranes incubated with horseradish peroxidase-conjugated goat anti-human Fc-specific (H+L) antibody (Jackson ImmunoResearch). Bound secondary conjugate was detected using Westernsure substrate, followed by scanning with a C-DiGit blot scanner (LI-COR).
Seldegs were also incubated in PBS (Lonza) at 4 °C (30 days) or 37 °C for 5 days, followed by analyses using a Superdex 200 5/150 GL column (GE Healthcare), 12% SDS–polyacrylamide gel electrophoresis and surface plasmon resonance (BIAcore).

Devanaboyina S.C., Khare P., Challa D.K., Ober R.J, & Ward E.S. (2017). Engineered clearing agents for the selective depletion of antigen-specific antibodies. Nature Communications, 8, 15314.

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Serum Stability of MOG-Seldeg-PS Protein

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MOG-Seldeg-PS was incubated in PBS at 4°C for 30 days or 37°C for 5 days, followed by analyses using a Phenomenex Yarra 3 μm SEC-3000 column (Phenomenex, 00H-4513-K0). For serum stability assays, endogenous IgGs were depleted from human serum (Sigma-Aldrich) by passage through protein G-Sepharose (GE Healthcare). MOG-Seldeg-PS was incubated in serum at a concentration of 400 nM at 37°C for 3 or 5 days as described previously.²⁶ (link) Following incubation, proteins were immunoprecipitated using agarose beads coupled with goat anti-human Fc-specific antibody (Sigma-Aldrich). Bound proteins were detected by immunoblotting with HRP-conjugated goat anti-human IgG (H+L) antibody (Jackson ImmunoResearch, West Grove, PA, USA). The immunoreactive bands were detected using WesternSure substrate (Thermo Fisher Scientific), followed by scanning with a C-DiGit blot scanner (LI-COR, Lincoln, NE, USA).

Sun W., Khare P., Wang X., Challa D.K., Greenberg B.M., Ober R.J, & Ward E.S. (2020). Selective Depletion of Antigen-Specific Antibodies for the Treatment of Demyelinating Disease. Molecular Therapy, 29(3), 1312-1323.

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