Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumamoto, Japan). Human recombinant IL-6 and IL-17A, and human neutralizing antibodies to IL-6 (aIL-6) and IL-17A (aIL-17A) were obtained from R&D Systems (Minneapolis, MN, USA). The monoclonal mouse anti-human IL-6 antibody (ab9324) was purchased from Abcam (Shanghai, China). Enzyme-linked immunosorbent assay (ELISA) kits for IL-17A, IL-6, IL-10, IL-1β, PGE2, and TGF-β; fluorescence-activated cell sorting (FACS) human antibodies including anti-CD4-FITC, anti-IL-17A-APC, anti-Foxp3-PE, anti-CD25-APC, and their corresponding anti-mouse IgG1 K-PE/APC; and the Annexin V apoptosis detection kit were all purchased from eBioscience (San Diego, CA, USA). Antibodies against JAK2, p-JAK2, STAT3, p-STAT3, p-AKT, AKT, cyclin D2, P27, and GAPDH were purchased from Santa Cruz Biotech (Santa Cruz, CA, USA). Real-time reverse transcription-polymerase chain reaction (RT-PCR) reagents were obtained from Takara (Beijing, China).Rituximab was purchased from Novartis (Basel, Switzerland). Doxorubicin and Ara-C were obtained from Pfizer (Shanghai, China).
Rt pcr reagents
Manufactured by Takara Bio
Sourced in China, Japan
RT-PCR reagents are a set of chemical compounds used in the reverse transcription-polymerase chain reaction (RT-PCR) technique. RT-PCR is a laboratory method for amplifying and detecting specific RNA sequences. The reagents provided by Takara Bio facilitate the conversion of RNA to complementary DNA (cDNA) and the subsequent PCR amplification of the cDNA.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd4 fitc, by Thermo Fisher Scientific (1 mentions)
Anti foxp3 pe, by Thermo Fisher Scientific (1 mentions)
Anti cd25 apc, by Thermo Fisher Scientific (1 mentions)
Anti il 17a apc, by Thermo Fisher Scientific (1 mentions)
Annexin 5 apoptosis detection kit, by Thermo Fisher Scientific (1 mentions)
P jak2, by Santa Cruz Biotechnology (1 mentions)
P akt, by Santa Cruz Biotechnology (1 mentions)
Doxorubicin, by Pfizer (1 mentions)
Ara c, by Pfizer (1 mentions)
Ab9324, by Abcam (1 mentions)
Ail 17a, by R&D Systems (1 mentions)
Rituximab, by Novartis (1 mentions)
Cyclin d2, by Santa Cruz Biotechnology (1 mentions)
P stat3, by Santa Cruz Biotechnology (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
Skbr3, by American Type Culture Collection (1 mentions)
Mcf 10a, by American Type Culture Collection (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Mda mb 468, by American Type Culture Collection (1 mentions)
2 protocols using rt pcr reagents
1
Evaluating Cytokine Modulation in Cell Assays
2
Metastatic Breast Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human breast cancer cell lines, MDA-MB-231, MDA-MB-468, ZR-75-30, T474D, MCF-7, ZR-75-30, and SK-BR-3, and one normal breast epithelial cell line, namely MCF-10A, were obtained from the American Type Culture Collection (Manassas, VA, USA).
MDA-MB-231HM and MDA-MB-231BO cell lines were established by subclone selection procedure in our institute. The MDA-MB-231HM cell line has a high potential to metastasize to the lung, its establishment has been described previously15 (link) and MDA-MB-231BO is more likely to metastasize to the bone. Cells were routinely maintained in the recommended medium supplemented with 10% fetal bovine serum, 100 U/mL of penicillin, and 100 μg/mL of streptomycin. The cultures were incubated at 37°C in a humidified 5% CO2 atmosphere. Cell culture medium and fetal bovine serum were purchased from Thermo and Invitrogen (Carlsbad, CA, USA). Real-time polymerase chain reaction (RT-PCR) reagents were from TaKaRa Bio Inc. (Kusatsu, Japan).
MDA-MB-231HM and MDA-MB-231BO cell lines were established by subclone selection procedure in our institute. The MDA-MB-231HM cell line has a high potential to metastasize to the lung, its establishment has been described previously15 (link) and MDA-MB-231BO is more likely to metastasize to the bone. Cells were routinely maintained in the recommended medium supplemented with 10% fetal bovine serum, 100 U/mL of penicillin, and 100 μg/mL of streptomycin. The cultures were incubated at 37°C in a humidified 5% CO2 atmosphere. Cell culture medium and fetal bovine serum were purchased from Thermo and Invitrogen (Carlsbad, CA, USA). Real-time polymerase chain reaction (RT-PCR) reagents were from TaKaRa Bio Inc. (Kusatsu, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!