Fmoc leu oh

Manufactured by Mimotopes

Sourced in China, Australia

Fmoc-Leu-OH is a chemical compound used in the synthesis of peptides and proteins. It is a protected amino acid, where the amino group is blocked with the Fmoc (fluorenylmethyloxycarbonyl) protecting group, and the carboxyl group is in the free acid form. This compound is commonly used as a building block in solid-phase peptide synthesis.

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Lab products found in correlation

Acetonitrile, by Merck Group (2 mentions) Fmoc dab ivdde oh, by Chem-Impex (2 mentions) Fmoc thr tbu oh, by Mimotopes (2 mentions) Fmoc d phe oh, by Chem-Impex (2 mentions) 0.22 m syringe filter, by Sartorius (1 mentions) Trifluoroacetic acid, by Merck Group (1 mentions) Triisopropylsilane, by Merck Group (1 mentions) Colistin sulfate, by Merck Group (1 mentions) Polymyxin b sulfate, by Merck Group (1 mentions) Hydrazine, by Merck Group (1 mentions) 1 1 1 3 3 3 hexafluoro 2 propanol hfip, by Chem-Impex (1 mentions) Fmoc ala oh, by Mimotopes (1 mentions)

2 protocols using fmoc leu oh

Synthesis of Polymyxin Lipopeptide Antibody

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Anti-polymyxin B mouse IgM antibody (clone 45) was obtained from Thermo
Fisher Scientific (Rockford, Illinois, USA). Polymyxin B (sulfate), colistin
(sulfate), triisopropylsilane (TIPS), trifluoroacetic acid, (TFA) and
diphenylphosphorylazide (DPPA), Streptavidin were purchased from Sigma-Aldrich
(Sydney, New South Wales, Australia). Piperidine and diisopropylethylamine
(DIPEA) were obtained from Auspep (Melbourne, Victoria, Australia).
Fmoc-Dab(Boc)-OH was purchased from Try-lead Chem (Hangzhou, Zhejiang,China).
Fmoc-Thr(tBu)-OH and Fmoc-Leu-OH were from Mimotopes (Melbourne, Australia).
Fmoc-Dab(ivDde)-OH, Fmoc-D-Phe-OH, Fmoc-Dap-OH, Fmoc-D-Dap-OH and
1H-Benzotriazolium-1-[bis(dimethylamino)methylene]-5-chloro
hexafluoro-phosphate-(1-),3-oxide (HCTU) were purchased from Chem-Impex
International (Wood Dale, Illinois, USA). Fmoc-Thr(tBu)-TCP-Resin was obtained
from Intavis Bioanyltical Instruments (Köln, Cologne,Germany).
Dimethylformamide (DMF), methanol (MeOH), diethyl ether, dichloromethane (DCM),
hydrochloric acid and acetonitrile were purchased from Merck (Melbourne,
Victoria, Australia). Polymyxin lipopeptide stock solutions were prepared with
Milli-Q water (Millipore, North Ryde, New South Wales, Australia) and filtered
using 0.22-µm syringe filters (Sartorius, Melbourne, Victoria,
Australia). Solutions were stored at 4°C for up to one month.^{57 (link)}

Velkov T., Yun B., Schneider E.K., Azad M.A., Dolezal O., Morris F.C., Nation R.L., Wang J., Chen K., Yu H.H., Chen L., Thompson P.E., Roberts K.D, & Li J. (2016). A novel chemical biology approach for mapping of polymyxin-lipopeptide antibody binding epitopes. ACS infectious diseases, 2(5), 341-351.

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Synthesis of Peptides with Fmoc Chemistry

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The general synthetic methodology used for the synthesis of the peptides has been previously described in detail elsewhere by our group.^{43 (link)} The materials and synthesis protocol used are also presented below.
Piperidine, diisopropylethylamine (DIPEA) and trifluoroacetic acid (TFA) were purchased from Auspep (Melbourne, Australia). Fmoc-Dab(Boc)-OH, was purchased from Try-lead Chem (Hangzhou, China). Fmoc-d-Phe-OH, Fmoc-Dab(ivDde)-OH, 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) and 1H-benzotriazolium-1-[bis(dimethylamino)methylene]-5-chloro hexafluoro phosphate-(1-),3-oxide (HCTU) were purchased from Chem-Impex International (Wood Dale, IL, USA). Fmoc-Ala-OH, Fmoc-Leu-OH and Fmoc-Thr(tBu)-OH were purchased from Mimotopes (Melbourne, Australia). Dimethylformamide (DMF), methanol (MeOH), diethyl ether, dichloromethane (DCM), hydrochloric acid (HCl) and acetonitrile were purchased from Merck (Melbourne, Australia). Fmoc-Thr(tBu)-TCP-Resin was purchased from Intavis Bioanayltical Instruments (Germany). Triisopropylsilane (TIPS), 1,2-ethanedithiol (EDT), diphenylphosphorylazide (DPPA) and hydrazine were obtained from Sigma-Aldrich (Castle Hill, Australia).

Jiang X., Patil N.A., Azad M.A., Wickremasinghe H., Yu H., Zhao J., Zhang X., Li M., Gong B., Wan L., Ma W., Thompson P.E., Yang K., Yuan B., Schreiber F., Wang L., Velkov T., Roberts K.D, & Li J. (2021). A novel chemical biology and computational approach to expedite the discovery of new-generation polymyxins against life-threatening Acinetobacter baumannii. Chemical Science, 12(36), 12211-12220.

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