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Dl glutamic acid

Manufactured by Cambridge Isotopes
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DL-Glutamic acid is a chemical compound that is commonly used as a laboratory reagent. It is a racemic mixture of the naturally occurring L-glutamic acid and its mirror image, D-glutamic acid. DL-Glutamic acid is a colorless, crystalline solid that is soluble in water and various organic solvents. It is often used in biochemical and analytical applications as a buffering agent or a component in culture media.

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3 protocols using dl glutamic acid

1

Quantitative Metabolite Analysis Protocol

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Formic acid (FA), methanol (MeOH), dimethylsulfoxid (DMSO) and acetonitrile (ACN) of analytical grade were from Fisher Scientific (Pittsburgh, PA, USA). Water was of ultrapure grade (EMD Millipore Co., Billerica, MA, USA). Deuterated standards {Fumaric acid (2,3-D2, 98% purity), DL-Glutamic acid (2,4,4-D3, 98% purity), L-Tryptophan (indole-D5, 98% purity) and Nicotinic acid (D4, 98% purity)} were from Cambridge Isotope Laboratories (Cambridge Isotope Laboratories Inc., Tewksbury, MA, USA). Deuterated Anthranilic acid (3,4,5,6-D4, 98% purity), Kynurenic acid (3,4,6,7,8-D5, 98% purity), Kynurenine (D4, 98% purity), Quinolinic acid (4,5,6-D3, 99% purity), 3-Hydroxy-DL-kynurenine (5,8,8-D3, 98% purity), 3-Hydroxyanthranilic acid (4,6-D2, 98% purity) were from Buchem (Buchem BV, Apeldoorn, The Nederlands). Deuterated Picolinic acid (99% purity) was from Acros Organics (Thermo Fisher Sci, NJ, USA). Commercial calibration solutions for the MS device were from Thermo Scientific (Thermo Fisher Sci., San José, CA, USA).
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2

Comprehensive Lipid and Metabolite Analysis

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PC(12:0/13:0), PE(12:0/13:0), SM(d18:1/12:0) and Cer(d18:1/12:0) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Stable isotope labeled references were purchased from Cambridge Isotope Laboratories (Andover, MA, USA), including: Glycine (2-13C, 15N), L-Alanine (2,3,3,3-D4), L-Valine (D8), L-Leucine (5,5,5-D3), L-Methionine (methyl-D3), L-Phenylalanine (ring-13C6), L-Tyrosine (ring-13C6), L-Aspartic acid (2,3,3-D3), DL-Glutamic acid (2,4,4-D3), L-Ornithine: HCl (5,5-D2), L-Citrulline (5,5-D2) and L-Arginine (5-13C, 4,4,5,5-D4). Deuterium-labeled carnitine and acylcarnitines (NSK-B set) were purchased from Cambridge Isotope Laboratories (Tewksbury, MA, USA), including 2H3-Acetylcarnitine (C2), 2H3-Propionylcarnitine (C3), 2H3-Butyrylcarnitine (C4), 2H9-Isovalerylcarnitine (C5), 2H3-Octanoylcarnitine (C8), 2H9-Myristoylcarnitine (C14), and 2H3-Palmitoylcarnitine (C16). HPLC-grade ammonium acetate was purchased from Sigma-Aldrich (St. Louis, MO, USA), and HPLC-grade formic acid and acetonitrile were purchased from Thermo Fisher Scientific (Waltham, MA, USA). All solutions were prepared by LC-MS ultra-pure water.
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3

Quantitative Metabolite Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Formic acid (FA), methanol (MeOH), dimethylsulfoxid (DMSO) and acetonitrile (ACN) of analytical grade were from Fisher Scientific (Pittsburgh, PA, USA). Water was of ultrapure grade (EMD Millipore Co., Billerica, MA, USA). Deuterated standards {Fumaric acid (2,3-D2, 98% purity), DL-Glutamic acid (2,4,4-D3, 98% purity), L-Tryptophan (indole-D5, 98% purity) and Nicotinic acid (D4, 98% purity)} were from Cambridge Isotope Laboratories (Cambridge Isotope Laboratories Inc., Tewksbury, MA, USA). Deuterated Anthranilic acid (3,4,5,6-D4, 98% purity), Kynurenic acid (3,4,6,7,8-D5, 98% purity), Kynurenine (D4, 98% purity), Quinolinic acid (4,5,6-D3, 99% purity), 3-Hydroxy-DL-kynurenine (5,8,8-D3, 98% purity), 3-Hydroxyanthranilic acid (4,6-D2, 98% purity) were from Buchem (Buchem BV, Apeldoorn, The Nederlands). Deuterated Picolinic acid (99% purity) was from Acros Organics (Thermo Fisher Sci, NJ, USA). Commercial calibration solutions for the MS device were from Thermo Scientific (Thermo Fisher Sci., San José, CA, USA).
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