Ab100736

Glycated hemoglobin (HbA_1c) has been validated as a reliable marker to measure glycemic control in mice (Feng et al., 2019 (link); Han et al., 2008 (link); Dan et al., 1997 (link)). Whole blood HbA_1c and plasma levels of TAG and prolactin were measured. HbA_1c was measured in whole undiluted blood collected in EDTA tubes using a commercial HbA_1c assay kit (Crystal Chem, Elk Grove Village, IL, USA). Plasma TAG was measured by preparing a 1:5 dilution using the Cayman Chemical kit. Plasma prolactin was measured in a 1:20 dilution of plasma using a commercially available ELISA kit (ab100736, Abcam, MA, USA). All assays were performed following the manufacturers’ instructions.

Immediately after acute sleep deprivation, mice were anaesthetized with 2–5% isoflurane and whole blood was collected by inferior vena cava puncture and coagulated at room temperature for 1 h. The serum was isolated by centrifugation at 6000 rcf, 10 min, at 4°C, and stored at −80°C until use. Serum corticosterone was quantified by a mouse corticosterone ELISA kit according to manufacturer instructions (ADI-900-097; Enzo Life Science, Farmingdale, NY, USA). Serum prolactin level was quantified by a mouse prolactin ELISA kit according to the manufacturer’s instructions (ab100736; Abcam, Waltham, MA, USA).