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Zgesgp pulse program

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The ZGESGP pulse program is a multidimensional NMR technique developed by Bruker. It is designed to acquire and process experimental data for nuclear magnetic resonance (NMR) spectroscopy applications. The core function of this pulse program is to enable the efficient acquisition and analysis of NMR signals.

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Advance 600 mhz nmr spectrometer, by Bruker (1 mentions) 1.7 mm cryogenic probe, by Bruker (1 mentions)

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Bruker pulse programs Zgesgp

3 protocols using zgesgp pulse program

1

NMR Sample Preparation for Aqueous Reactions

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To the reaction mixture was added ~300 mg of solid KOH (NaHS∙xH2O in the case of Mo-promoted reactions) in order to precipitate out any metal ions as their hydroxides (or sulfides in the case of Mo). This was mixed thoroughly. The resulting thick suspension was transferred to a 1.5 mL plastic microtube and centrifuged at 10 000 rpm for 20 minutes. To 600 µL of the supernatant was added 100 µL of 0.05 M solution of internal standard (DSS-Na in D2O). The resulting solution was analysed by NMR using the Bruker ZGESGP pulse program.
Varma S.J., Muchowska K.B., Chatelain P, & Moran J. (2018). Native iron reduces CO2 to intermediates and end-products of the acetyl CoA pathway. Nature ecology & evolution, 2(6), 1019-1024.
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2

NMR Sample Preparation for Aqueous Reactions

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To the reaction mixture was added ~300 mg of solid KOH (NaHS∙xH2O in the case of Mo-promoted reactions) in order to precipitate out any metal ions as their hydroxides (or sulfides in the case of Mo). This was mixed thoroughly. The resulting thick suspension was transferred to a 1.5 mL plastic microtube and centrifuged at 10 000 rpm for 20 minutes. To 600 µL of the supernatant was added 100 µL of 0.05 M solution of internal standard (DSS-Na in D2O). The resulting solution was analysed by NMR using the Bruker ZGESGP pulse program.
Varma S.J., Muchowska K.B., Chatelain P, & Moran J. (2018). Native iron reduces CO2 to intermediates and end-products of the acetyl CoA pathway. Nature ecology & evolution, 2(6), 1019-1024.
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3

NMR Characterization of Purified Metabolite

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NMR experiments were performed on a Bruker Advance 600-MHz NMR spectrometer equipped with a 1.7 mm cryogenic probe.
The 1 H NMR water signal from the polar fraction was suppressed by means of excitation sculpting (Bruker ZGESGP pulse program). Additional 13 C NMR spectra were acquired for each sample. 1 H-1 H COSY, 1 H-13 C HSQC, 1 H-13 C HMBC and 1 H-15 N HMBC experiments was only carried out for the purified metabolite sample.
Chemical shifts (expressed in ppm) of 1 H NMR spectra were referenced to the solvent peaks δ H 4.79 for D 2 O.
3-((3-Aminopropyl)amino)-4-hydroxybenzoic acid (M211; APAH):
Thomas M., Stuani L., Darii E., Lechaplais C., Pateau E., Tabet J., Salanoubat M., Saaidi P., & Perret A. (2019). De novo structure determination of 3-((3-aminopropyl)amino)-4-hydroxybenzoic acid, a novel and abundant metabolite in Acinetobacter baylyi ADP1. Metabolomics, 15(3).
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