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Nss spectral imaging

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The NSS Spectral Imaging is a laboratory equipment designed to capture and analyze spectral data from samples. It provides high-resolution spatial and spectral information, enabling detailed characterization of materials and substances.

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Lab products found in correlation

Jsm 6610, by Thermo Fisher Scientific (3 mentions) Jsm 6610, by JEOL (2 mentions) O coumaric acid, by Merck Group (1 mentions) Coumarin, by Merck Group (1 mentions) Jsm 6610 microscope, by JEOL (1 mentions) Vacuum desk 5, by Denton (1 mentions)

6 protocols using nss spectral imaging

1

Characterization of Medicinal Leaf Material

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The M. laevigata leaves were collected from the medicinal plant garden at the Faculty of Pharmacy (UFG) in Goiania, Goias, Brazil (S16°40’33” W49°14’39”) and identified by Dr. Jose Realino de Paula of the Universidade Federal de Goiás (UFG).
The leaves were dried in an air-circulating stove (Solab Equipamentos para Laboratório Ltda, Piracicaba, SP, Brasil) at 40°C and crushed in a knife mill TE-625 (Tecnal Ltda, Piracicaba, SP, Brazil). The drug material was characterized by particle size analysis (Scanning Electron Microscopy JEOL, JSM-6610, equipped with EDS -Energy Dispersed Spectroscopy-, Thermo scientific NSS Spectral Imaging. Akishima, Japan), calculation of volatile content (moisture balance MB 35, Ohaus Inc., Pine Brook, NJ, USA), swelling index,[17 ] and by the thin layer chromatographic (TLC)[18 ] profile using coumarin and o-coumaric acid (Sigma, St. Louis, MO, USA) as reference substances.
Pinto M.V., Oliveira E.M., Martins J.L., de Paula J.R., Costa E.A., da Conceição E.C, & Bara M.T. (2017). Obtaining a Dry Extract from the Mikania laevigata Leaves with Potential for Antiulcer Activity. Pharmacognosy Magazine, 13(49), 76-80.
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2

Ultrastructural Analysis of M. abscessus

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M. abscessus subsp. massiliense colonies grown on MH agar were removed from the plates and exposed to 3.8 or 7.6 μg/mL of Polydim-I or 0.5 μg/mL of clarithromycin for 24 h. As a negative control, the colonies were incubated with PBS alone. After incubation, the cells were fixed with modified Karnovsky solution (paraformaldehyde 1% and glutaraldehyde 3% in 0.07 M cacodylate buffer, pH 7.2) for 30 min at 4°C. The fixative solution was subsequently removed, and the samples were dehydrated through increasing concentrations of ethanol solution (30%, 50%, 70%, 90%, and 100%) for 10 min, followed by acetone and hexamethyldisilazane (HMDS) (V/V) for an additional 5 min. The final dehydration step was performed with HMDS for 5 min. After the samples were completely dry, a thin layer of gold was deposited using a Denton Vacuum Desk V apparatus. The images were obtained using a Jeol JSM – 6610 microscope (Jeol, Japan) equipped with energy dispersive spectroscopy—EDS (Thermo scientific NSS Spectral Imaging).
das Neves R.C., Trentini M.M., de Castro e Silva J., Simon K.S., Bocca A.L., Silva L.P., Mortari M.R., Kipnis A, & Junqueira-Kipnis A.P. (2016). Antimycobacterial Activity of a New Peptide Polydim-I Isolated from Neotropical Social Wasp Polybia dimorpha. PLoS ONE, 11(3), e0149729.
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3

Spray Drying Herbal Extract Optimization

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To obtain the dried extracts, a laboratory-scale spray dryer model MSD 1.0 (Labmaq do Brasil Ltda., Ribeirão Preto, SP, Brazil) with a concurrent flow regime was used. A drying pressure air spray of 60 psi, air flow sprinkler of 35 L/min, drying air flow of 1.0 m3/min, inlet temperature of 90°C, and diameter atomizing nozzle of 1.2 mm were used. The extract flow rates tested were 2 and 6 mL/min. Drying was performed without any drying adjuvants and with colloidal silicon dioxide at 15 and 30%.
The physicochemical parameters of the dried extract measured were the chemical profile using TLC and determination of coumarin and o-coumaric acid by HPLC. Process yield, volatile content, water activity (Testo 650, AG, Lenzkirch, Germany), and particle morphology (Scanning Electron Microscopy JEOL, JSM-6610, equipped with EDS -Energy Dispersed Spectroscopy-, Thermo scientific NSS Spectral Imaging. Akishima, Japan) were measured.
Pinto M.V., Oliveira E.M., Martins J.L., de Paula J.R., Costa E.A., da Conceição E.C, & Bara M.T. (2017). Obtaining a Dry Extract from the Mikania laevigata Leaves with Potential for Antiulcer Activity. Pharmacognosy Magazine, 13(49), 76-80.
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4

Scanning Electron Microscopy Analysis of Root Canal

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After fixation in buffered glutaraldehyde, specimens were sectioned to expose the
root canal. Two longitudinal grooves were made on each side along the root
length using a diamond-coated disk (KG Sorensen Ind. Com., São Paulo, Brazil) at
low rotation and air/water spray, making sure that the internal part of the
canal was not touched. Next, a chisel was used to split the samples carefully
along the buccolingual axis. Samples were dehydrated in 70%, 95%, and 100%
alcohol solutions. The roots were kept for 30 min in each solution, which was
refreshed every 10 min. CO2 critical point drying (Autosamdri®, 815,
Series A) was performed before sputter-coating with gold (Denton Vacuum, Desk
V).
The images were obtained using SEM images (Jeol, JSM 6610, equipped with EDS,
Thermo Scientific NSS Spectral Imaging, Tokyo, Japan). The surface of the root
canal was examined throughout the length of the two samples of each root,
beginning in the cervical third and ending in the apical. The qualitative
evaluations were carried out at magnifications 1,600X and 5,000X to describe the
effect of PDT on the dentinal structure of the root canal.
Alves D.R., Decucio D.D., de Alencar A.H., Estrela C.R., de Souza J.B., Pinheiro A.L, & Estrela C. (2022). Effect of low-power diode laser on infected root canals. Brazilian Dental Journal, 33(3), 8-17.
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5

Scanning Electron Microscopy Imaging of Vesicles

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SEM images were obtained in
a JEOL scanning electron microscope,
model JSM 6610, equipped with a Thermo Scientific NSS Spectral Imaging.
Images were acquired at a spot size set at 40 mm, work distance at
14 mm, and with an accelerated voltage of 4 keV. A volume of 20 μL
of the freshly prepared vesicles dispersed in acetone was deposited
onto a glass substrate, and the solvent was evaporated in an oven
at 40 °C for 48 h. Samples were placed in a copper sample holder,
and metallization was carried out in a Bal-Tec MED 020 sputtering
chamber.
Albino de Souza G., de Castro Bezerra F, & Martins T.D. (2020). Photophysical Properties of Fluorescent Self-Assembled Peptide Nanostructures for Singlet Oxygen Generation. ACS Omega, 5(15), 8804-8815.
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6

Fractured Sample SEM Analysis

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Randomly selected fractured samples from the FS test were subjected to SEM analysis. The fixed samples on metal stubs were sputtered with gold (one cycle of 120 s) under vacuum in a sputtering device (Denton Vacuum, Desk V, Moorestown, EUA). The surfaces were analyzed by SEM (JEOL, JSM-6610; Thermo Scientific NSS Spectral Imaging, São Paulo, Brazil), focusing on the fracture features as well as the integrity and homogeneity along the interfaces between the glass fibers and resin matrix.
Almeida L.N., Mendes G.A.M., Favarão I.N., Kasuya A.V.B., Borges M.G., Menezes M.S, & Fonseca R.B. (2018). Influence of preheating and post-curing on a novel fiber-reinforced composite post material. Brazilian oral research, 32.
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