Multicycle software wincycle 32

Manufactured by Phoenix Pharmaceuticals

MultiCycle software WinCycle 32 is a software tool designed for data analysis and processing. It is capable of performing multi-cycle analysis of experimental data. The software provides functionality for organizing, visualizing, and analyzing data from various experimental techniques.

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Lab products found in correlation

Facscan, by BD (1 mentions)

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Multicycle software (89 citations) MultiCycle (15 citations) WinCycle software (7 citations)

2 protocols using multicycle software wincycle 32

Cell Cycle Analysis of BEAS-2B Cells

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Normal epithelium cell BEAS-2B were grown in the presence of 9 mM M-PFC NPs for 24 h. Trypsinized cells were harvested and washed in cold PBS(3×), then isolated by centrifugation (1273 g for 5 min). Single cell suspensions were added to pre-chilled 75% ethanol and fixed at -20°C overnight. The cells were rinsed with phosphate buffer, resuspended, then post-fixed with Cell Cycle Assay KIT (Biyuntian Company, Shanghai, China). Flow cytometry was performed using a FACScan (Becton Dickinson, San Jose, CA) equipped with 488 nm and 633 nm lasers. Samples were gated to exclude debris and clumps, and electronic compensation was used to remove residual spectral overlap. The experiment was repeated in triplicate and data were analyzed using MultiCycle software WinCycle 32 (Phoenix Flow Systems, Inc., San Diego, CA).

Wu L., Wen X., Wang X., Wang C., Sun X., Wang K., Zhang H., Williams T., Stacy A.J., Chen J., Schmieder A.H., Lanza G.M, & Shen B. (2018). Local Intratracheal Delivery of Perfluorocarbon Nanoparticles to Lung Cancer Demonstrated with Magnetic Resonance Multimodal Imaging. Theranostics, 8(2), 563-574.

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Cell Cycle Analysis by Flow Cytometry

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For cell cycle analysis, attached and non-adherent cells treated in 6-well plates were collected within the media, rinsed with PBS, fixed in 70% ethanol overnight, and hypotonically lysed in 0.5 ml of propidium iodide buffer (0.5mg/ml propidium iodide, 0.1% sodium citrate, 0.05% Triton X-100). Samples were analyzed on a Beckman-Coulter (Fullerton, CA) EPICS XL flow cytometer with laser output adjusted to deliver 15 MW at 488 nm. Ten thousand cells were counted. Cell cycle analysis was then performed using MultiCycle software WinCycle 32 (Phoenix Flow Systems, San Diego, CA).

Hargraves K.G., He L, & Firestone G.L. (2015). Phytochemical regulation of the tumor suppressive microRNA, miR-34a, by p53-dependent and independent responses in human breast cancer cells. Molecular carcinogenesis, 55(5), 486-498.

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