Sphereclone ods

Manufactured by Phenomenex

Sourced in Italy

Sphereclone ODS is a reversed-phase high-performance liquid chromatography (HPLC) column. It utilizes a silica-based stationary phase with octadecylsilane (ODS) bonding for the separation of a variety of organic compounds.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

SphereClone (24 citations) Sphereclone ODS column (4 citations) SphereClone™ C18 ODS (2 citations) SphereClone ODS (2) column (2 citations)

4 protocols using sphereclone ods

Analysis of Organic Compounds by UV-Vis, NMR, and LC-MS

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ultraviolet–visible (UV–Vis) spectra were recorded on a Jasco V-730 spectrophotometer (Lecco, Italy).
Nuclear magnetic resonance (NMR) spectra were recorded at 400 MHz on a Bruker instrument (Milan, Italy).
HPLC analyses were performed on an Agilent 1100 binary pump instrument (Agilent Technologies, Milan, Italy) equipped with a UV–Vis detector, using an octadecylsilane-coated column, 250 mm × 4.6 mm, 5 µm particle size (Phenomenex SphereClone ODS, Bologna, Italy) at 0.7 mL/min, and the following gradient: 0.1% formic acid (eluent a)/methanol (eluent b): 40% b, 0–10 min; from 40 to 80% b, 10–47.5 min. The detection wavelength was set at 280 nm.
Liquid chromatography–mass spectrometry (LC–MS) analysis was performed on an HPLC 1100 VL series instrument (Agilent Technologies) with an electrospray ionization source in positive ion mode (ESI+). An Agilent Eclipse XDB-C18, 150 mm × 4.60 mm, 5 µm (Agilent Technologies) was used, with the same eluent used for the HPLC analysis at a flow rate of 0.4 mL/min. Mass spectra were registered under the following conditions: nebulizer pressure 50 psi; drying gas (nitrogen) flow 10 L/min, at 350 °C; and capillary voltage 4000 V.

Micillo R., Pistorio V., Pizzo E., Panzella L., Napolitano A, & d’Ischia M. (2017). 2-S-Lipoylcaffeic Acid, a Natural Product-Based Entry to Tyrosinase Inhibition via Catechol Manipulation. Biomimetics, 2(3), 15.

+ Open protocol

+ Expand

Analytical HPLC and Preparative HPLC Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

HPLC analyses were performed on an Agilent 1100 instrument equipped with a UV detector set at 254 nm. An octadecylsilane-coated column, 250 mm × 4.6 mm, 5 μm particle size (Phenomenex Sphereclone ODS) at 0.7 mL/min was used with the following gradient: 1.5% formic acid (eluant a)/methanol (eluant b) from 5 to 90% b, 0–45 min. Preparative HPLC was carried out on an instrument coupled with a UV detector set at 280 nm using an Econosil C18 (10 micron, 22 × 250 mm), at 15 mL/min, and 1.5% formic acid: methanol 85:15 as the eluant. UV-vis spectra were recorded on a Jasco V-730 Spectrophotometer.

Micillo R., Panzella L., Iacomino M., Prampolini G., Cacelli I., Ferretti A., Crescenzi O., Koike K., Napolitano A, & d’Ischia M. (2017). Eumelanin broadband absorption develops from aggregation-modulated chromophore interactions under structural and redox control. Scientific Reports, 7, 41532.

+ Open protocol

+ Expand

Antioxidant Potential of Melanin-like Pigments

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gelatin type A from porcine skin (gel strength ~300 g bloom), 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium-chloride (DMTMM), chitosan, glacial acetic acid, 2,2-diphenyl-1-picrilhydrazyl (DPPH), ferric chloride (III) hexahydrate, and 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ) were purchased from Sigma Aldrich (Milano, Italy) and used without any further purification. Phosphate buffer saline (PBS) 10× was purchased from VWR. DHICA and MeDHICA were prepared according to a procedure previously developed [36 (link),37 (link)].
The UV–Vis spectra were recorded on a Jasco V-730 Spectrophotometer (Lecco, Italy).
HPLC analyses were performed on an Agilent 1100 binary pump instrument (Agilent Technologies, Milan, Italy) equipped with a SPD-10AV VP UV–visible detector using an octadecylsilane-coated column, 250 mm × 4.6 mm, 5 μm particle size (Phenomenex Sphereclone ODS, Bologna, Italy) at 0.7 mL/min. Detection wavelength was set at 300 nm. Eluant system: 1% formic acid:acetonitrile, 85:15 v/v.

Alfieri M.L., Pilotta G., Panzella L., Cipolla L, & Napolitano A. (2020). Gelatin-Based Hydrogels for the Controlled Release of 5,6-Dihydroxyindole-2-Carboxylic Acid, a Melanin-Related Metabolite with Potent Antioxidant Activity. Antioxidants, 9(3), 245.

+ Open protocol

+ Expand

Spectrophotometric Analysis of Organic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

3,4-dihydroxy-L-phenylalanine (L-DOPA), potassium ferricyanide, sodium bicarbonate, sodium dithionite, hexamethylenediamine (HMDA), 1,2-ethylenediamine 1,4-butanediamine, 1,12dodecanediamine, butylamine, 2,2-diphenyl-1-picrilhydrazyl (DPPH), ferric chloride (III) hexahydrate and trispyridylhydrazine (TPTZ) were purchased by Sigma-Aldrich. The UV-vis spectra were recorded on a Jasco V-730 Spectrophotometer. HPLC analyses were performed on a Agilent 1100 binary pump instrument equipped with and a SPD-10AV VP UV-visible detector using an octadecylsilane-coated column, 250 mm × 4.6 mm, 5 μm particle size (Phenomenex Sphereclone ODS) at 0.7 mL/min (detection wavelength was established at 300 nm). Eluent system: Gradient of wateracetonitrile from t5 to 45 min from 40 to 70% of acetonitrile. Positive Reflectron MALDI spectra were recorded on a AB Sciex TOF/TOF 5800 instrument using 2,5-dihydroxybenzoic acid as the matrix. Substrates (quartzes, borosilicate coverslips) were cleaned by soaking in a solution of H2SO4/30% H2O2 5:1 overnight, then cleaned with distilled water. Quartz substrates were dipped in the mixtures and after 24 h were rinsed with distilled water, sonicated in methanol/water solution 1:1 v/v, and airdried. The coated substrates thus obtained were analysed by ultraviolet-visible (UV-vis) spectrophotometry.

Argenziano R., Alfieri M.L., Panzella L., Napolitano A., & d’Ischia M. (2020). Antioxidant activity of film forming systems based on melanins from 5,6-dihydroxyindole derivatives.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!