McCoy’s 5A medium (#16600–082), RPMI-1640 (#11875–093), DMEM (#11965–092), Penicillin-Streptomycin (#15140), Trypsin-EDTA (#25200–056) were obtained from Invitrogen (Life Technologies). Dulbecco’s Phosphate Buffered Saline (PBS) (#21–030-CV) was obtained from Corning Cellgro. Fetal Bovine Serum (FBS) (#100-602) was obtained from Gemini Biosciences. 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) (#M2128) and Doxorubicin Hydrochloride (DOX; #D1515 and #44583 for fluorescence studies) was obtained from Sigma Aldrich. Antibodies against MDR1 (D3H1Q), MRP1 (D708N), ABCG2 (D5V2K), phospho-p38MAPK (D3F9), p38MAPK (D13E1), phospho-p44/42 MAPK (Erk1/2) (D13.14.4E), p44/42 MAPK (Erk1/2) (137F5), phospho-SAPK-JNK (81E11), SAPK-JNK (9252) and GAPDH (14C10) were obtained from Cell Signaling Technology. Anti-4-Hydroxynonenal antibodies were obtained from Abcam (#ab46545). Anti-rabbit secondary antibodies (#170–6515) and protein standard for SDS-PAGE (#161–0375) were obtained from Bio-Rad. Annexin V-Alexa Fluor 488 (#A13201) and 7-aminoactinomycin D (7-AAD) (#A1310) were obtained from Molecular Probes, Invitrogen. Fidarestat was obtained from Livwel Therapeutics Inc, CA. USA.
Protein standard for sds page
Manufactured by Bio-Rad
Protein standard for SDS-PAGE is a mixture of proteins with known molecular weights used to determine the molecular weights of unknown protein samples in SDS-PAGE. It serves as a reference to compare and identify the molecular weights of the proteins in the sample.
Automatically generated - may contain errors
Lab products found in correlation
Doxorubicin hydrochloride dox, by Merck Group (2 mentions)
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt, by Merck Group (2 mentions)
Dulbecco s phosphate buffered saline, by Corning (2 mentions)
Anti rabbit secondary antibody, by Bio-Rad (2 mentions)
Trypsin edta, by Thermo Fisher Scientific (2 mentions)
Gapdh 14c10, by Cell Signaling Technology (1 mentions)
Annexin 5 alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
7 aminoactinomycin d 7 aad, by Thermo Fisher Scientific (1 mentions)
Mccoy s 5a medium, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Phospho nf κb, by Cell Signaling Technology (1 mentions)
3 nitrotyrosine, by Abcam (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
2 protocols using protein standard for sds page
1
Cell Line Characterization and Cytotoxicity Assay
2
Endothelial Cell Growth and Treatment
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