Total RNA was isolated with a miRNeasy Micro kit (QIAGEN) or Trizol reagent (Thermo Fisher Scientific). RNA was reverse-transcribed using the miRCURY LNA Universal RT microRNA cDNA synthesis kit (Exiqon). Mature miR-181a expression levels were assessed by quantitative RT-PCR using the miRCURY LNA UniRT PCR primer for miR-181a-5p (Exiqon) and Power SYBR® Green PCR Master Mix (Thermo Fisher Scientific). U6 or RNU48 (Exiqon) were used as internal control to normalize miR-181a expression. In some experiments, a TaqMan MicroRNA Reverse Transcription kit and TaqMan MicroRNA Assays (Thermo Fisher Scientific) were used for reverse transcription and quantitative RT-PCR of miR-181a and sno202. Expression levels were displayed as 2−ΔΔCt.
Mircury lna unirt pcr primer for mir 181a 5p
Manufactured by Qiagen
The MiRCURY LNA UniRT PCR primer for miR-181a-5p is a laboratory equipment product designed for real-time quantitative PCR (RT-qPCR) analysis of the miRNA miR-181a-5p. It is part of the MiRCURY LNA Universal RT microRNA PCR system.
Automatically generated - may contain errors
Lab products found in correlation
Trizol reagent, by Thermo Fisher Scientific (4 mentions)
Taqman microrna assay, by Thermo Fisher Scientific (2 mentions)
U6 or rnu48, by Qiagen (2 mentions)
Mircury lna universal rt microrna cdna synthesis kit, by Qiagen (2 mentions)
Power sybr green pcr master mix, by Thermo Fisher Scientific (2 mentions)
Taqman microrna reverse transcription kit, by Thermo Fisher Scientific (2 mentions)
Mirneasy micro kit, by Qiagen (2 mentions)
2 protocols using mircury lna unirt pcr primer for mir 181a 5p
1
Quantitative Analysis of miR-181a Expression
2
Quantitative Analysis of miR-181a Expression
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Total RNA was isolated with a miRNeasy Micro kit (QIAGEN) or Trizol reagent (Thermo Fisher Scientific). RNA was reverse-transcribed using the miRCURY LNA Universal RT microRNA cDNA synthesis kit (Exiqon). Mature miR-181a expression levels were assessed by quantitative RT-PCR using the miRCURY LNA UniRT PCR primer for miR-181a-5p (Exiqon) and Power SYBR® Green PCR Master Mix (Thermo Fisher Scientific). U6 or RNU48 (Exiqon) were used as internal control to normalize miR-181a expression. In some experiments, a TaqMan MicroRNA Reverse Transcription kit and TaqMan MicroRNA Assays (Thermo Fisher Scientific) were used for reverse transcription and quantitative RT-PCR of miR-181a and sno202. Expression levels were displayed as 2−ΔΔCt.
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