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Q100 70 7

Manufactured by Sutter Instruments
Sourced in United States

The Q100-70-7.5 is a laboratory instrument manufactured by Sutter Instruments. It is designed to provide a controlled voltage output for various applications in scientific research and experimentation. The device specifications and technical details are available upon request.

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2 protocols using q100 70 7

1

Fabrication of Quartz Nanopipettes

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Example 2

A P-2000 CO2 laser puller (Sutter Instrument, Novato, Calif.) was used to pull quartz capillaries (Q100-70-7.5, Sutter Instrument, Novato, Calif.) to inner diameters that range from 20 nm to 35 nm. All capillaries were cleaned with piranha solution (3:1 v/v H2SO4:30% H2O2) prior to pulling. After fabrication, all pipettes were imaged using scanning electron microscopy and scanning transmission electron microscopy (Quanta FEG 600F, FEI, Hillsboro, Oreg.). Pipettes were back-filled with PBS using a MicroFil needle (World Precision Instruments, Sarasota, Fla.) and centrifuged before use in experiments.

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2

Quartz Glass Pipette Fabrication

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Pipettes used in the experiments were pulled from quartz glass capillaries (Q100-70-7.5, Sutter Instrument, Novato, CA, USA) with a CO2-laser puller (P2000, Sutter Instrument, Novato, CA, USA). Typical pulling parameters used in the experiment were heat = 690, Fil = 4, Vel = 45, Del = 160, and Pul = 190. Pipettes were then characterized by scanning electron microscopy (SEM; FEI Quanta-FEG, Hillsboro, OR). Electron micrographs are shown in Section SI-1. The typical inner and outer radii of pipettes are 50 and 70 nm, respectively, with a half-cone angle of ~7.5°.
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