Rabbit anti fak c 20

Antibodies used for western blot analysis: mouse anti-GFP (1:1000; Proteintech, #50430-2-AP), rabbit anti-pFAK Y576 (1:200; Santa Cruz, #sc-16563), rabbit anti-pFAK Y397 (1:200; Novus, #NBPI-60837), rabbit anti-pPaxillin Y31 (1:200; Santa Cruz, #sc-14035), mouse anti-FAK (1:1000; Proteintech, #66258-I-1g), mouse anti-Paxillin (1:5000; BD Biosciences, #610051), rabbit anti-FLAG (1:1000; Proteintech, #80010-1-RR).
Antibodies used in immunofluorescence experiments: mouse anti-FAK 4.47 (1:1000; Millipore, #05-537), mouse anti-FAK (1:1000; Proteintech, #66258-I-1g), rabbit anti-FAK C-20 (1:200; Santa Cruz, #sc-558), rabbit anti-pFAK Y576 (1:200; Santa Cruz, #sc-16563), rabbit anti-pFAK Y397 (1:200; Novus, #NBPI-60837) goat anti-Talin C-20 (1:750; Santa Cruz, #sc-7534), mouse anti-Paxillin (1:750; R&D Systems, #AF4259), mouse anti-Vinculin (1:1000; Sigma, #V9131), rabbit anti-Vinculin (1:2000; Proteintech, #26520-I-AP), mouse anti-av Integrin H-2 (1:100; Santa Cruz, #sc-376156), rabbit anti-pPaxillin Y31 (1:200; Santa Cruz, #sc-14035), mouse anti-p-Tyr (pY20) (1:200; Santa Cruz, #sc-508), rat anti-active β1-Integrin 9EG7 (1:700; BD Bioscences, #550531), mouse anti-p130 Cas 35B.1A4 (1:500; Santa Cruz, #sc-20029), mouse anti-Tensin C-2 (1:1000; Santa Cruz, #sc-376367).

Recombinant human VEGF₁₆₅ (Ala27-Arg191, no. 293-VE), human Sema3C (Gly21-Ser738, no. 5570-S3), and mouse Sema3C (Gln24-Ser741, no. 1728-S3) were from R&D Systems. Recombinant human IgG-Fc (009-000-008) was purchased from Jackson ImmunoResearch. Antibodies: rat anti-Sema3C (clone 238835) and goat anti-Nrp-1 (AF566) are from R&D Systems; rabbit anti-pY397FAK, rabbit anti-pT180/pY182-p38MAPK (Invitrogen); rabbit anti-FAK (C-20), and goat anti-Nrp-1 (C-19) are from Santa Cruz; mouse anti-p38MAPK (L53F8), rabbit anti-AKT, rabbit anti-pS473AKT, and rabbit anti-cleaved Caspase-3 (Asp175) are from Cell Signaling; pTyr118 paxillin (NEB), rat anti-CD34 (NCL-END; Novocastra); rat anti-CD31 (clone Mec13.3) and rat anti-VE-cadherin are from BD Biosciences; mouse anti-vinculin (V9131) and anti-actin smooth muscle-Cy3 are from Sigma; rabbit anti-GFAP (DAKO), rabbit anti-NG2 (Millipore), rabbit anti-collagen IV (Cosmo Bio).