To determine whether anti-rPoMPO Abs inhibits MPO activity, the supernatant of HKLs was incubated alone, or with anti-rPoMPO Abs (1:1000) for 45 min. The activity of MPO was measured by the MPO colorimetric activity assay kit (Nanjing Jiancheng Co., Ltd., China) according to the manufacturer’s instructions. Finally, the absorbance was measured at 460 nm to evaluate the MPO activity.
Mpo colorimetric activity assay kit
Manufactured by Nanjing Jiancheng
Sourced in China
The MPO colorimetric activity assay kit is a laboratory reagent used to measure the enzymatic activity of myeloperoxidase (MPO) in biological samples. It provides a quantitative measurement of MPO levels through a colorimetric reaction.
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3 protocols using mpo colorimetric activity assay kit
1
Anti-rPoMPO Abs Inhibit MPO Activity
2
Lung Tissue MPO, MIP2, and KC Analysis
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The homogenates of lung tissue were centrifuged to obtain supernatant for the following experimental detections. The activity of MPO was measured by the MPO colorimetric activity assay kit (Nanjing Jiancheng Co., Ltd., China) according to the manufacturer's instructions. Finally, the absorbance was measured at 460 nm to evaluate the MPO activity. The levels of MIP2 and KC in supernatant were measured using an ELISA kit (Biosource International), and all procedures were performed in strict accordance with the kit instructions.
3
Lung Tissue MPO Activity Assay
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MPO activity was measured in lung tissues as an index of neutrophil accumulation and lung inflammation as previously reported (Zhou et al. 2019 (link)). Briefly, lung tissues were ground into homogenates and centrifuged, and the supernatants were collected. Measurement of MPO activity was implemented utilising the MPO colorimetric activity assay kit (Nanjing Jiancheng Co., Ltd., China) in accordance with the manufacturer’s protocol.
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