For brain tissue analysis, animals were sublethally anesthetized by intraperitoneal injection of pentobarbital. Mice were then perfused transcardially with sterile cold saline solution until the blood was completely washed out. Brains were quickly removed and placed in cold medium and then dissociated using a Neural Tissue Dissociation kit (Miltenyi Biotec) according to the instructions of the manufacturer. Single-cell suspensions were analyzed using the following antibodies: CD45.2-PerCP (Biolegend), CD11b-PE-cy7 (BD Bioscience), F4/80-PE (BD Bioscience), Ly6C-FITC (BD Bioscience), and CD115-PerCP (Biolegend) and subjected to flow cytometry analysis.
Cd115 percp
Manufactured by BioLegend
CD115-PerCP is a fluorochrome-conjugated monoclonal antibody that binds to the CD115 (CSF-1R) surface receptor. CD115 is expressed on monocytes, macrophages, and osteoclasts. The PerCP fluorescent label allows for the detection and enumeration of CD115-positive cells using flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Neural tissue dissociation kit, by Miltenyi Biotec (1 mentions)
Cd45.2 percp, by BioLegend (1 mentions)
F4 80 pe, by BD (1 mentions)
Ly6c fitc, by BD (1 mentions)
Cd11b pe cy7, by BD (1 mentions)
Isotype control, by BioLegend (1 mentions)
Sytox red, by Thermo Fisher Scientific (1 mentions)
Isotype control, by Thermo Fisher Scientific (1 mentions)
F4 80 pe, by BioLegend (1 mentions)
Ly6g pe, by BioLegend (1 mentions)
G csf, by R&D Systems (1 mentions)
Gro α, by R&D Systems (1 mentions)
Human il 8, by R&D Systems (1 mentions)
Gr 1 pe, by BioLegend (1 mentions)
Cd11b allophycocyanin, by BioLegend (1 mentions)
D luciferin, by GoldBio (1 mentions)
Cit h3, by Abcam (1 mentions)
Sytox green, by Thermo Fisher Scientific (1 mentions)
Mcp 1, by R&D Systems (1 mentions)
2 protocols using cd115 percp
1
Brain Tissue Immune Cell Analysis
2
Detailed Immunological Reagents Inventory
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Sources of Ab for the indicated applications were as follows: for flow cytometry, Ly6G-PE, F4/80-PE, Gr1-PE, CD11b-peridinin-chlorophyll-protein (PerCP), CD115-PerCP, CD244.2-PerCP, CD11b-allophycocyanin, and isotype controls (BioLegend); for immunohistochemistry, CD31, Ly6G, neutrophil elastase, human nucleoli, cit-H3 (Abcam), PNAd (Santa Cruz Biotechnology), MPO (R&D Systems), isotype controls (Thermo Fisher Scientific), and Alexa Fluor 488– and Alexa Fluor 594–conjugated secondary Ab (Life Technologies); and for neutralization, Ly6G (clone 1A8), isotype controls (BioXcell), and human IL-8, MCP-1, GROα, pan-GRO, and G-CSF (R&D Systems). Sources of chemicals were as follows: Sytox Green and Sytox Red (Life Technologies); PMA and DAPI (Sigma-Aldrich); CI-amidine and GSK484 (Cayman Chemical); recombinant DNase I and collagenase type II (Worthington Biochemical Corp.); and D-luciferin (Gold Biotechnology).
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