The whole-cell lysate or the immunocomplexes were separated by 8 to 12% SDS-PAGE and transferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore, Billerica, MA, USA). Anti-KAT2A (Cell Signaling, Danvers, MA, USA, 1:1000, #3305), anti-E2F1 (Invitrogen, Waltham, MA, USA; Thermo Fisher Scientific, Waltham, MA, USA, 1:1000, MA1-23202), anti-H3K9ac (Cell Signaling Technology, 1:1000, 9649S), anti-UBE2C (Invitrogen, Thermo Fisher Scientific, 1:1000, PA5-27223), anti-β-actin (Beyotime Biotechnology, Shanghai, China, 1:1000, AA128), and horseradish peroxidase (HRP)-conjugated secondary antibodies (anti-mouse and anti-rabbit IgG) (Beyotime Biotechnology, 1:2000, A0208, A0216) antibodies were used to detect each protein. Bands were detected using BeyoECL Star chemiluminescence substrate (Beyotime Biotechnology, P0018AM).
Anti kat2a
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-KAT2A is a primary antibody that specifically binds to the KAT2A protein. KAT2A is a histone acetyltransferase that plays a role in regulating gene expression.
Automatically generated - may contain errors
Lab products found in correlation
Ripa buffer, by Thermo Fisher Scientific (2 mentions)
Anti β actin, by Beyotime (1 mentions)
Anti h3k9ac, by Cell Signaling Technology (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Total histone extraction kit, by Epigentek (1 mentions)
Anti lamin a c, by Cell Signaling Technology (1 mentions)
Anti phospho acc ser79, by Cell Signaling Technology (1 mentions)
Anti acc, by Cell Signaling Technology (1 mentions)
Ponceau s solution, by Merck Group (1 mentions)
Protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)
Anti cox 4, by Abcam (1 mentions)
Anti acetyl lysine, by Cell Signaling Technology (1 mentions)
Anti succinyllysine, by PTM Biolabs (1 mentions)
Anti malonyl lysine, by PTM Biolabs (1 mentions)
Anti h2b, by Cell Signaling Technology (1 mentions)
Trichostatin a, by Cayman Chemical (1 mentions)
Anti hsp60, by Abcam (1 mentions)
Anti sirt5, by Cell Signaling Technology (1 mentions)
Anti β tubulin, by Cell Signaling Technology (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
2 protocols using anti kat2a
1
Immunoblot Analysis of Protein Modifications
2
Histone Extraction and Protein Analysis
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Cells and tissues are lysed and homogenized with RIPA buffer (Thermo Fisher Scientific, Cat. #89900) containing protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific, Cat. # PI78447), 5 M trichostatin A (Cayman Chem, Cat. #89730), and 5 mM nicotinamide (Sigma-Aldrich, Cat. #72340). Histones were extracted from cells and tissues using a total histone extraction kit (EpiGentek, Cat. #OP-0006). Before SDS-PAGE, protein samples were heated at 70°C for 5 min. Antibodies used are listed as follows: anti-β-tubulin (Cell Signaling Technology, Cat. #2128), anti-SIRT5 (Cell Signaling Technology, Cat. #8782), anti-acetyl-lysine (Cell Signaling Technology, Cat. #9814), anti-malonyl-lysine (PTM Biolabs, Cat. #PTM-901), anti-succinyl-lysine (PTM Biolabs, Cat. # PTM-401), anti-glutaryl-lysine (PTM Biolabs, Cat. #PTM-1151), anti-ACC (Cell Signaling Technology, Cat. #3676), anti-phospho-ACC (Ser79) (Cell Signaling Technology, Cat. #3661), anti-H2B (Cell Signaling Technology, Cat. #12364), anti-KAT2A (Cell Signaling Technology, Cat. #3305), anti-HSP60 (Abcam, Cat. #ab59457), anti-Lamin A/C (Cell Signaling Technology, Cat. #2032), anti-COX IV (Abcam, Cat. #ab16056), and anti-GAPDH (Cell Signaling Technology, Cat. #5174). Ponceau S solution (Sigma-Aldrich, Cat. #P7170) was used for protein staining.
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