Finnigan tsq 7000

Pac-525 (Ac-KWRRWVRWI-NH₂, molecular weight: 1,426.70 Da), KSL-W (KKVVFWVKFK-NH₂, molecular weight: 1,307.69 Da), and KSL (KKVVFKVKFK-NH₂, molecular weight: 1,249.65 Da) were synthesized and used in our study. A high-efficiency solid-phase peptide synthesis was performed by ChinaPeptides Co., Ltd. in Shanghai, China, by using an automatic peptide synthesizer (Symphony; Protein Technologies, Tucson, AZ). The purified peptide was characterised using analytical high performance liquid chromatography (HPLC; Shimadzu, Kyoto, Japan) and mass spectrometry (MS, Finnigan TSQ 7000; Thermo, Waltham, MA) with purity of over 99%. All the synthesized peptides were freeze-dried and stored at −20°C.

The peptides TKII-12 (HVLKNRRLTWEY) and TKII-10 (RNPDGDAKPW) were synthesized by a high-efficiency solid-phase method using an automatic peptide synthesizer (Symphony; Protein Technologies, Tucson, AZ). The end product was characterized by high-performance liquid chromatography (HPLC, analytical; Shimadzu, Kyoto, Japan) and mass spectrometry (MS; Finnigan TSQ 7000; Thermo, Waltham, MA). To verify whether the effect of TKII-12 was sequence-dependent, we scrambled the amino acid sequence of TKII-12 and synthesized TKII-12S (KRYLTHNVRWLE). These peptides were freeze-dried and stored at −20°C until used. Immediately before use, the peptides were dissolved in phosphate-buffered saline (PBS). Both these peptides were water-soluble and stable in aqueous solutions.