Anti lbpa z plbpa

Primary antibodies used were obtained from the following companies: anti-APPL1 (3858) and anti-Rab7 (9367) from Cell Signaling; anti-EEA1 (612006) from BD Biosciences; anti-M6PR (ab2733), anti-RILP (ab140188) and anti-LAMP1 (ab24170) from Abcam; anti-PIKfyve (AF7885) from R&D systems; anti-LBPA (Z-PLBPA) from Echelon; anti-Golgin97 (A-21270), anti-Hrs (PA5-27491) and anti-Rab11 (71-5300) from Thermo Fischer Scientific; anti-Rabenosyn-5 (222218-1-AP) from Proteintech; anti-Rabankyrin5 (11321) and anti-VPS51 (HPA039650) from Sigma-Aldrich; anti-GFP (132002), anti-β-actin (251003; 1:10,000 dilution for Western blotting), anti-Rab5 (108011), anti-Syntaxin 6 (110062), anti-Syntaxin 13 (110132), anti-Vti1a (165003), anti-VAMP4 (136002), anti-Syntaxin 7 (110072), anti-Syntaxin 8 (110083), anti-Vti1b (164002), and anti-VAMP8 (104302) from Synaptic Systems. For Western blotting, the appropriate primary antibodies were used at a dilution of 1:1000 (or as otherwise stated). Alexa Fluor 488-, Cy3-, or Cy5-conjugated goat anti-mouse (115–545–166, 115–165–146, 115–175–166) and goat anti-rabbit IgG (111–545–144, 111–165–144, 111–175–144) were from Jackson ImmunoResearch Laboratories, and HRP-conjugated goat anti-mouse (STAR117P) and goat anti-rabbit (5196–2504) were obtained from Bio-Rad.

The following primary antibodies were used for Western blot and ICC: anti‐GFP (GTX113617, GeneTex), anti‐Alix/AIP1 (ABC40, Merck), anti‐CD81 (sc‐166029, Santa Cruz Biotechnology), rabbit anti‐rat Rab5 (C8R1, Cell Signaling Technology), rabbit anti‐rat Rab7 (D95F2, Cell Signaling Technology), anti‐Rab11 (D4F5, Cell Signaling Technology), rabbit anti‐rat EEA1 (C45B10, Cell Signaling Technology), anti‐LBPA (Z‐PLBPA, Echelon Biosciences), anti‐CHC (D3C6, Cell Signaling Technology). The following chemical compounds and growth factors were used: BAPTA‐AM (Tocris), Genistein (Tocris), EGTA (Tocris), bFGF (Peprotech), Poly‐l‐Lysine (Sigma), Tetrodotoxin (TTX) (Abcam), Tetraethylammonium chloride (TEA) (Abcam).