Sp5 up right confocal microscope

Manufactured by Definiens

The SP5 upright confocal microscope is a versatile imaging platform designed for high-resolution fluorescence microscopy. It features a powerful confocal scanning system that enables optical sectioning and 3D reconstruction of biological samples. The SP5 allows for the visualization and analysis of a wide range of specimens, such as cells, tissues, and small organisms.

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Lab products found in correlation

Axio scan z1, by Zeiss (2 mentions) Developer xd2, by Definiens (2 mentions) Zen blue software v3, by Zeiss (1 mentions) Dmi1 inverted microscope, by Leica (1 mentions)

2 protocols using sp5 up right confocal microscope

Imaging Brain Metastases Quantification

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Immunofluorescence images were acquired with a Leica SP5 up‐right confocal microscope ×5, ×10, ×20, ×40, and ×63 objectives and analyzed with ImageJ software and Definiens developer XD 2.5. Immunohistochemistry images were captured with the Zen Blue Software v3.1 (Zeiss), and whole slides were acquired with a slide scanner (AxioScan Z1, Zeiss). For histological quantification of brain metastases at endpoint (5 weeks after intracardiac inoculation of cancer cells), only lesions showing solid and compact distribution of cancer cells were considered as established metastases.

Zhu L., Retana D., García‐Gómez P., Álvaro‐Espinosa L., Priego N., Masmudi‐Martín M., Yebra N., Miarka L., Hernández‐Encinas E., Blanco‐Aparicio C., Martínez S., Sobrino C., Ajenjo N., Artiga M., Ortega‐Paino E., Torres‐Ruiz R., Rodríguez‐Perales S., Soffietti R., Bertero L., Cassoni P., Weiss T., Muñoz J., Sepúlveda J.M., González‐León P., Jiménez‐Roldán L., Moreno L.M., Esteban O., Pérez‐Núñez Á., Hernández‐Laín A., Toldos O., Ruano Y., Alcázar L., Blasco G., Fernández‐Alén J., Caleiras E., Lafarga M., Megías D., Graña‐Castro O., Nör C., Taylor M.D., Young L.S., Varešlija D., Cosgrove N., Couch F.J., Cussó L., Desco M., Mouron S., Quintela‐Fandino M., Weller M., Pastor J, & Valiente M. (2022). A clinically compatible drug‐screening platform based on organotypic cultures identifies vulnerabilities to prevent and treat brain metastasis. EMBO Molecular Medicine, 14(3), e14552.

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Multimodal Microscopy Imaging Protocol

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Images were acquired with a Leica SP5 upright confocal microscope with ×10, ×20, ×40 and ×63 objectives and analyzed with Fiji software 1.0 and Definiens developer XD 2.5. Immunohistochemistry images were captured with Zen Blue Software v3 (Zeiss), and whole slides were acquired with a slide scanner (AxioScan Z1, Zeiss). Oncospheres were imaged with a Leica DMi1 inverted microscope.

Monteiro C., Miarka L., Perea-García M., Priego N., García-Gómez P., Álvaro-Espinosa L., de Pablos-Aragoneses A., Yebra N., Retana D., Baena P., Fustero-Torre C., Graña-Castro O., Troulé K., Caleiras E., Tezanos P., Muela P., Cintado E., Trejo J.L., Sepúlveda J.M., González-León P., Jiménez-Roldán L., Moreno L.M., Esteban O., Pérez-Núñez Á., Hernández-Lain A., Mazarico Gallego J., Ferrer I., Suárez R., Garrido-Martín E.M., Paz-Ares L., Dalmasso C., Cohen-Jonathan Moyal E., Siegfried A., Hegarty A., Keelan S., Varešlija D., Young L.S., Mohme M., Goy Y., Wikman H., Fernández-Alén J., Blasco G., Alcázar L., Cabañuz C., Grivennikov S.I., Ianus A., Shemesh N., Faria C.C., Lee R., Lorigan P., Le Rhun E., Weller M., Soffietti R., Bertero L., Ricardi U., Bosch-Barrera J., Sais E., Teixidor E., Hernández-Martínez A., Calvo A., Aristu J., Martin S.M., Gonzalez A., Adler O., Erez N, & Valiente M. (2022). Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism. Nature Medicine, 28(4), 752-765.

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