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Jfc 1100 sputter

Manufactured by JEOL
Sourced in United States

The JFC-1100 sputter is a versatile sputtering device designed for depositing thin films onto various substrates. It utilizes a sputtering process to create uniform and high-quality coatings. The core function of the JFC-1100 is to deposit thin films with precise control over the thickness and deposition rate.

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3 protocols using jfc 1100 sputter

1

Morphological Characterization of Polyphenol Microcapsules

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The morphological characteristics of the microcapsules containing the polyphenols were identified using scanning electron microscopy (SEM) with an JEOL JSM-6610LV microscope (JEOL Inc., USA) with an acceleration voltage of 10 kV. Before imaging, each sample was sputter-coated with gold into a JEOL JFC-1100 sputter for 3 min. All the samples were processed and visualized at room temperature (~20 °C). Digital images were captured using Quartz PCI imaging software v8 (Quartz Imaging Corp., Vancouver, BC, Canada).
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2

Spray-dried A. indica Microcapsules Analysis

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The optimal treatment was submitted to scanning electron microscopy (SEM) analysis. Previously, the treatment was dehydrated and pulverized using a Spray-dryer (Buchi, Mini spray-dryer B-290, Switzerland). The equipment was adjusted to the following conditions: feed flow at 6 mL/min, the inlet temperature of 160 °C, and drying airflow at 40 m3/h. The powder was recovered and stored in hermetically sealed bags at room temperature until SEM analysis.
The morphological characteristics of the microcapsules containing the A. indica extracts were identified by scanning electron microscopy (SEM) using a JEOL JSM-6610LV Microscope (JEOL Inc., Peabody, MA, USA) with an acceleration voltage of 10 kV. Before imaging, each sample was sputter-coated with gold into a JEOL JFC-1100 sputter for 3 min. All the samples were processed and visualized at room temperature (20 °C). Digital images were captured using Quartz PCI imaging software v8 (Quartz Imaging Corp., Vancouver, British Columbia, Canada).
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3

SEM Analysis of Tissue Biofilms

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Acquired tissue samples were prepared for SEM assessment within 30 min after excision. Mucosa fragments were fixed using a solution of 2.5% glutaraldehyde in 0.2 M cacodylate buffer (pH 7.4). Each fragment was immersed in this solution for 24 h in 4°C. Next, after thorough rinsing in 0.2 M cacodylate buffer, which was exchanged several times, the samples were fixed again, this time in 2% osmium tetroxide, for 3 h. Next, the tissues were dehydrated in rising concentrations of ethyl alcohol and absolute acetone and dried in CO 2 . The dried specimens were mounted on plates using silver glue and sputter-coated with gold using a JFC-1100 sputter (Jeol, Tokyo, Japan). The casts were examined in a JSM 35-CF scanning electron microscope (Jeol) at 25 kV.
Each biofilm was assessed independently by three examiners, who had no contact with the patients and who were blind to the result of the clinical assessment. The basic criterion for diagnosing a biofilm was the presence of spherical and rod-shaped structures, surrounded by a matrix and situated on the epithelium.
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