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4 protocols using sodium acetate

1

Tart Cherry Jam Preparation

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Tart cherries were purchased at a local market, pits were removed, and flesh was used for the preparation of jams. The following chemicals were used: Potassium chloride, sodium acetate, hydrochloric acid, methanol, sodium carbonate, sodium bisulfite, Folin-Ciocalteu reagent were purchased from Kemika (Zagreb, Croatia). Trolox, 2,2’-azinobis (3-ethylbenzothiazoline sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazil (DPPH) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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2

Antioxidant Capacity Evaluation Protocol

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In this study, the following reagents and standards were used: aluminium chloride, quercetin dihydrate, gallic acid monohydrate, potassium persulfate, Trolox, 2,2-azinobis(3-ethylbenzothiazoline sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazil (DPPH), 2,4,6-tripyridyl-s-triazine (TPTZ) were obtained from Sigma-Aldrich (St. Lois, MO, USA); HPLC standards (gallic and caffeic acid, (+)-catechin hydrate, (−)-epicatechin, rutin hydrate, quercetin) were purchased from Sigma-Aldrich Chemie Gmbh (Steinheim, Germany), and malvidin 3-glucoside from Extrasynthese (Genay, France). Folin–Ciocalteu reagent, sodium nitrite, sodium carbonate, sodium hydroxide, potassium bisulphite, sodium acetate, potassium chloride and hydrochloric acid were purchased from Kemika (Zagreb, Croatia); sodium acetate trihydrate, ferric chloride hexahydrate, and ammonium acetate were purchased from Gram-Mol (Zagreb, Croatia); copper(II) chloride from Acros Organics (New Jersey, NJ, USA); HPLC grade methanol and neocuproine from Merck (Darmstadt, Germany) and phosphoric acid (HPLC grade) were obtained from Fluka (Buchs, Switzerland).
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3

Comprehensive Chemical Inventory for Research

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Chemicals and standards used in this study were obtained as follows: gallic acid monohydrate, quercetin dihydrate, aluminum chloride, potassium persulfate, Trolox, 2,2 -azinobis(3-ethylbenzothiazoline sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazil (DPPH), 2,4,6-tripyridyl-s-triazine (TPTZ), gallic acid, caffeic acid, catechin, and epicatechin from Sigma-Aldrich (St. Lois, MO, USA); Folin-Ciocalteu reagent, sodium carbonate, sodium nitrite, sodium hydroxide, potassium bisulfite, potassium chloride, sodium acetate, and hydrochloric acid from Kemika (Zagreb, Croatia); sodium acetate trihydrate, ferric chloride hexahydrate, and ammonium acetate from Gram-Mol (Zagreb, Croatia); copper(II) chloride from Acros Organics (New Jersey, NJ, USA); HPLC grade methanol and neocuproine from Merck (Darmstadt, Germany) and phosphoric acid (HPLC grade) from Fluka (Buchs, Switzerland).
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4

Histidine-Albumin Interaction Analysis

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All the reagents used were of analytical grade. L-histidine (His) and human serum albumin (HSA) were obtained from Sigma-Aldrich (St. Louis, USA). 0.1 M acetate buffer containing 0.1 M sodium perchlorate pH = 4.6 (AB) was used. Acetic acid, sodium acetate, copper(II) nitrate and sodium perchlorate were from Kemika (Zagreb, Croatia). All the solutions were prepared with deaerated double deionized water from Millipore-MilliQ system (USA).
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