8 ohdg

Manufactured by Nanjing Jiancheng

Sourced in China

8-OHdG is a biomarker that measures oxidative DNA damage. It is a modified nucleoside that is released when DNA is damaged by reactive oxygen species. The 8-OHdG assay can be used to quantify the level of this biomarker in biological samples.

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Lab products found in correlation

Glutathione (gsh), by Nanjing Jiancheng (3 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) E004 1 1, by Nanjing Jiancheng (1 mentions) Cleaved caspase 3, by Abcam (1 mentions) Mda test kit, by Nanjing Jiancheng (1 mentions) Bcl 2, by Abcam (1 mentions) Bcl2 associated x (bax), by Abcam (1 mentions) Bca protein assay kit, by Beyotime (1 mentions)

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8-OHdG assay kit (2 citations) 8-OHdG ELISA kit (2 citations)

6 protocols using 8 ohdg

Assessing Oxidative Stress Markers in Mouse Brain

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Following sacrifice by cervical dislocation, the cerebral cortex and hippocampus were isolated from each mouse and were homogenized in 2% SDS containing a protease inhibitor cocktail (Sigma-Aldrich; Merck KGaA) and phosphatase inhibitors (Calbiochem; Merck KGaA). The homogenized mixes were centrifuged at 100,000 × g for 1 h at 4°C. The supernatant was stored as soluble fraction to detect ROS (cat. no. E004-1-1; Nanjing Jiancheng Bioengineering Institute), 8-OhdG (cat. no. H165; Nanjing Jiancheng Bioengineering Institute) and 3-NT (cat. no. 267-N3; R&D Systems, Inc.) according to the manufacturer's instructions (4 (link)).

Jiang H., Niu F., Zheng Y, & Xu Y. (2021). CART mitigates oxidative stress and DNA damage in memory deficits of APP/PS1 mice via upregulating β-amyloid metabolism-associated enzymes. Molecular Medicine Reports, 23(4), 280.

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Mequindox-Induced Oxidative Stress Model

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Mequindox (MEQ; C₁₁H₁₀N₂O₃, CAS No: 60875-16-3, purity 98%) was obtained from Beijing Zhongnongfa Pharmaceutical Co., Ltd. (Huanggang, China). SOD, GSH, 8-OHdG, MDA, and LDH kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Nrf-2 (D1Z9C) XP Rabbit mAb (12721T) and cleaved caspase 3 (Asp175) antibody (9661T) were purchased from Cell Signaling Technology (United States), and a reagent Western Blot Stripping Buffer (T7135A) was purchased from Takara (Japan). All other chemicals and reagents were of analytical grade and obtained from Sigma-Aldrich (St. Louis, MO, United States).

Liu Q., Lei Z., Guo J., Liu A., Lu Q., Fatima Z., Khaliq H., Shabbir M.A., Maan M.K., Wu Q., Dai M., Wang X., Pan Y, & Yuan Z. (2018). Mequindox-Induced Kidney Toxicity Is Associated With Oxidative Stress and Apoptosis in the Mouse. Frontiers in Pharmacology, 9, 436.

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Mequindox Oxidative Stress Evaluation

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Mequindox (C₁₁H₁₀N₂O₃, molecular weight 218.21 g/mol, CAS No: 60875-16-3, purity 98%) was obtained from Beijing Zhongnongfa Pharmaceutical Co. Ltd. (Huanggang, PR China). MDA, PCC, SOD, GSH and 8-OHdG kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, P.R. China). All other chemicals were purchased from Sigma (St. Louis, USA) unless otherwise stated.

Liu Q., Lei Z., Huang A., Wu Q., Xie S., Awais I., Dai M., Wang X, & Yuan Z. (2017). Toxic metabolites, MAPK and Nrf2/Keap1 signaling pathways involved in oxidative toxicity in mice liver after chronic exposure to Mequindox. Scientific Reports, 7, 41854.

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Germacrone-Induced Apoptosis Signaling

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In the present study, Germacrone (GM) (≥ 98%) was purchased from Nanjing Puyi Biological Technologies. 8oHdG and MDA test kits were obtained from the Nanjing Jiancheng Bioengineering Institute in Nanjing, China. Rabbit antibodies targeting proteins associated with apoptosis (Bcl-2, Bax, cleaved caspase-3, NF-κB, and COX-2) were purchased from Abcam (Cambridge, USA. Santa Cruz Biotechnology, Dallas, TX, USA). Rabbit tubulin and goat anti-rabbit IgG for peroxidase were acquired from Santa Cruz Biotechnology.

Yuan Y, & Shao L. (2024). Germacrone Protects against NF-κB-Mediated Inflammatory Signaling, Apoptosis, and Retinal Ganglion Cell Survival in a Rat Glaucoma Model. The Tohoku journal of experimental medicine.

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Antioxidant Biomarkers in Tissue Samples

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Tissue samples were harvested, homogenized in normal saline, and centrifuged at 4°C for 10 min at 859 g. The supernatant was collected to measure the indicators with kits per the manufacturer's protocols. The protein concentration was measured by using a BCA Protein Assay Kit (Beyotime, China). The kits were PC (Cat No. A087-1-2), MDA (Cat No. A003-1-2), 8-OhdG (Cat No. H165-1-2), SOD (Cat No. A00-1-2), CAT (Cat No. A00-1-1), GPx (Cat No. A005-1-2), and GSH (Cat No. A006-1-1) purchased from Jiancheng (Nanjing, China).

He L., Sui C., Li J., Yao Y., Li M., Wang R, & Zhu W. (2021). N-Terminal 5-Mer Peptide Analog P165 of Amyloid Precursor Protein Repairs Skin Photodamage Induced by UVB through the Nrf2 Signaling Pathway. Indian Journal of Dermatology, 66(5), 574.

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Lung Tissue Antioxidant and Oxidative Stress Analysis

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A portion of the tissue preparation was made from right lung tissue that was homogenized with normal saline, and the preparation was centrifuged at 2500 rpm at 4 °C for 10 min. The resultant supernatant was equilibrated with saline and used to determine the levels of proteins in the tissue homogenates. The levels of superoxide dismutase (SOD), glutathione (GSH), catalase (CAT), malondialdehyde (MDA), myeloperoxidase (MPO), and 8-hydroxydeoxyguanosine (8-OHdG) were determined using commercial kits (Nanjing Jiancheng, Nanjing, China). The determinations were performed strictly according to the manufacturer’s instructions.

Zhang N., Deng C., Zhang X., Zhang J, & Bai C. (2018). Inhalation of hydrogen gas attenuates airway inflammation and oxidative stress in allergic asthmatic mice. Asthma research and practice, 4, 3.

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