PS and VLX1570 were purchased from Selleck Chemicals, (Houston, Texas, TX, USA). This was dissolved in dimethylsulfoxide and stored at -80°C with being protected from light. We used those at the concentrations up to 200 nM.
Vlx1570
Manufactured by Selleck Chemicals
Sourced in United States
The VLX1570 is a laboratory instrument designed for industrial and scientific applications. It serves as a device for measuring and analyzing various properties and characteristics of samples. The VLX1570 provides accurate and reliable data to support research, development, and quality control processes.
Automatically generated - may contain errors
Lab products found in correlation
400 mhz spectrometer, by Bruker (1 mentions)
B ap15, by Selleck Chemicals (1 mentions)
Sq detector 2, by Waters Corporation (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Pr 619, by Merck Group (1 mentions)
Amd3100, by Selleck Chemicals (1 mentions)
Antibodies against β actin c4, by Santa Cruz Biotechnology (1 mentions)
Wp1130, by Merck Group (1 mentions)
Recombinant human cxcl12 sdf 1, by R&D Systems (1 mentions)
N acetylcysteine, by Merck Group (1 mentions)
N n dimethylthiourea, by Merck Group (1 mentions)
Mannitol, by Fujifilm (1 mentions)
Bortezomib, by Selleck Chemicals (1 mentions)
5 protocols using vlx1570
1
Compound storage and preparation
2
Analytical Procedures for Organic Compounds
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Solvents
were purchased
from commercial sources at high-performance LC (HPLC) or analytical
grade and used without purification. Analytical and preparative LC–MS
were performed using a Waters system equipped with a Waters 2545 Binary
Gradient Module, SecurityGuard ULTRA cartridges for EVO-C18 UHPLC
HPLC, a Kinetex 5 μM EVO C18 100 Å 100 × 3.0 mm column,
and a Waters SQ Detector 2. NMR spectra were recorded using a Bruker
400 MHz spectrometer using the deuterated solvent stated. Chemical
shifts (δ) are quoted in parts per million (ppm) and referenced
to the residual solvent peak. Reagents were purchased from Sigma-Aldrich
unless otherwise stated and used without further purification. b-AP15
and VLX1570 were purchased from Selleckchem and used as supplied without
further purification.
were purchased
from commercial sources at high-performance LC (HPLC) or analytical
grade and used without purification. Analytical and preparative LC–MS
were performed using a Waters system equipped with a Waters 2545 Binary
Gradient Module, SecurityGuard ULTRA cartridges for EVO-C18 UHPLC
HPLC, a Kinetex 5 μM EVO C18 100 Å 100 × 3.0 mm column,
and a Waters SQ Detector 2. NMR spectra were recorded using a Bruker
400 MHz spectrometer using the deuterated solvent stated. Chemical
shifts (δ) are quoted in parts per million (ppm) and referenced
to the residual solvent peak. Reagents were purchased from Sigma-Aldrich
unless otherwise stated and used without further purification. b-AP15
and VLX1570 were purchased from Selleckchem and used as supplied without
further purification.
3
Jurkat Leukemic T Cell Culture
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The Jurkat leukemic T cell line (clone E6.1) was from American Type Culture Collection (ATCC, catalog number TIB-152). Jurkat cells were cultured at 37°C in 5% CO2 in RPMI supplemented with 10% fetal bovine serum (FBS) (Hyclone), and DMEM, 10% fetal bovine serum, respectively). Control (CT) and Actin CytoFRET (ACT) reporter cell lines were generated as previously described (Larbret et al., 2013 (link)). Antibodies against β-actin (C4) were purchased from Santa Cruz Biotechnology, cofilin (D3F9), ERK2 (9108), profilin (C56B8), ubiquitin (P4D1), and phospho-ADF/cofilin (3312) were purchased from Cell Signaling Technology. Anti-gelsolin (ab11081) was from Abcam. Anti-thymosin β4 (AF6796) and recombinant human CXCL12/SDF-1 were from R&D Systems. WP1130, b-AP15 and PR-619 were from Merck. VLX1570 and AMD3100 were from Selleckchem. All other chemicals were from Merck. Plasmid DNA were transfected with Lipofectamine 2000 (Thermo Fischer Scientific).
4
Antioxidant Compound Preparation and Storage
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5
Bortezomib and VLX1570 Dissolution
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PS, Bortezomib and VLX1570 were purchased from Selleck Chemicals, (Houston, Texas, TX, USA). This was dissolved in dimethylsulfoxide and stored at -80°C with being protected from light. We used those at the concentrations up to 200 nM.
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