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Elisa kits for mouse il 4

Manufactured by R&D Systems
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Sourced in United States

The ELISA kits for mouse IL-4 from R&D Systems are designed to quantitatively measure mouse interleukin-4 (IL-4) in cell culture supernates, serum, and plasma.

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Lab products found in correlation

Cytometric bead array, by BD (1 mentions) Spectramax m2e microplate reader, by Molecular Devices (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions)

Close spelling variants of this product

ELISAs (144 citations) Mouse ELISA kits (102 citations) Mouse IL-4 (21 citations) IL-4 kit (3 citations) Mouse IL-4 ELISA kits (2 citations) IL-4 ELISA kits (2 citations)

2 protocols using elisa kits for mouse il 4

1

Cytokine and Chemokine Measurement by ELISA

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ELISA was used to measure cytokine/chemokine concentrations in culture media, lung tissue suspensions, and BALF according to the manufacturer’s instructions. Mouse interferon-γ (IFN-γ), IL-6, IL-10, and MCP-1 were detected using the BD Cytometric Bead Array (BD Biosciences). ELISA kits for mouse IL-4, IL-13, KC, human IL-8, and CTGF were provided by R&D Systems, for active TGF-β1 by BioLegend, and for mouse CTGF by Lifespan Biosciences.
Li F.J., Surolia R., Li H., Wang Z., Liu G., Kulkarni T., Massicano A.V., Mobley J.A., Mondal S., de Andrade J.A., Coonrod S.A., Thompson P.R., Wille K., Lapi S.E., Athar M., Thannickal V.J., Carter A.B, & Antony V.B. (2021). Citrullinated vimentin mediates development and progression of lung fibrosis. Science translational medicine, 13(585), eaba2927.
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2

Quantification of IL-4 Expression in Modified MSCs

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ELISA kits for mouse IL-4 (R&D system, Minneapolis, MN, United States) were used to quantify IL-4 expression by the genetically modified MSCs exposed to 1 μg/ml LPS (Sigma Aldrich, St. Louis, MO) or left untreated for 24 h culture. The manufacturers’ protocols were carefully followed. The optical densities were determined using SpectraMax M2e Microplate Readers (Molecular Devices, San Jose, CA, United States) set at 450 nm with wavelength correction set to 540 nm.
Zhang N., Utsunomiya T., Lin T., Kohno Y., Ueno M., Maruyama M., Huang E., Rhee C., Yao Z, & Goodman S.B. (2021). Mesenchymal Stem Cells and NF-κB Sensing Interleukin-4 Over-Expressing Mesenchymal Stem Cells Are Equally Effective in Mitigating Particle-Associated Chronic Inflammatory Bone Loss in Mice. Frontiers in Cell and Developmental Biology, 9, 757830.
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