Labchip gx hisens rna system

Manufactured by PerkinElmer

The LabChip GX HiSens RNA system is a lab equipment product from PerkinElmer designed for the analysis of RNA samples. It provides automated, high-throughput assessment of RNA quality and quantity. The system utilizes microfluidic technology to perform electrophoretic separation and detection of RNA molecules.

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Lab products found in correlation

Paxgene blood rna tube, by Preanalytix (2 mentions) Rneasy spin column, by Qiagen (1 mentions) Nanodrop, by Agilent Technologies (1 mentions) Nextseq 500 high output kit v2, by Illumina (1 mentions) Paxgene blood mirna kit, by Qiagen (1 mentions) Hiseq 2500, by Illumina (1 mentions) Qiacube, by Qiagen (1 mentions)

Close spelling variants of this product

LabChip GX system LabChip GX LabChip system

2 protocols using labchip gx hisens rna system

Longitudinal Transcriptome Profiling of TB

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Patient samples were collected prior to initiation of treatment (diagnosis), at weeks 2, 4, 8, 16 and 24 through treatment, and at 12 months after TB diagnosis (6 months after treatment completion), and from HC at baseline only. Venous blood (2·5ml) was collected into PAXgene Blood RNA Tubes (PreAnalytiX). Total RNA was extracted using RNeasy spin columns (Qiagen) and quantified by Nanodrop (Agilent). The LabChip GX HiSens RNA system (PerkinElmer) was used for quality assessment of samples processed by RNA-Seq.

van Doorn C.L., Eckold C., Ronacher K., Ruslami R., van Veen S., Lee J.S., Kumar V., Kerry-Barnard S., Malherbe S.T., Kleynhans L., Stanley K., Hill P.C., Joosten S.A., van Crevel R., Wijmenga C., Critchley J.A., Walzl G., Alisjahbana B., Haks M.C., Dockrell H.M., Ottenhoff T.H., Vianello E, & Cliff J.M. (2022). Transcriptional profiles predict treatment outcome in patients with tuberculosis and diabetes at diagnosis and at two weeks after initiation of anti-tuberculosis treatment. eBioMedicine, 82, 104173.

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Transcriptome profiling of TB-DM comorbidity

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Samples from TB patients (with and without DM) were collected at baseline, 2, and 6 months of treatment. Samples for the non-TB/non-DM control and DM only groups were collected at baseline. Total RNA from venous blood (2.5 mL) was collected in PAXgene Blood RNA Tubes (PreAnalytiX), extracted using a PAXgene blood miRNA kit (Qiagen) with the semi-automated QIAcube (Qiagen) and quantified using the LabChip GX HiSens RNA system (PerkinElmer). RNA-seq libraries were prepared using the Bioscientific NEXTflex-Rapid-Directional mRNA-seq sample preparation with the Caliper SciClone. Samples were sequenced using the NextSeq500 High Output kit V2 (Illumina) for 75 cycles. For all samples, RNA was sequenced by Illumina HiSeq 2500 at MedGenome in Bangalore, India.

Vinhaes C.L., Fukutani E.R., Santana G.C., Arriaga M.B., Barreto-Duarte B., Araújo-Pereira M., Maggitti-Bezerril M., Andrade A.M., Figueiredo M.C., Milne G.L., Rolla V.C., Kristki A.L., Cordeiro-Santos M., Sterling T.R., Andrade B.B, & Queiroz A.T. (2024). An integrative multi-omics approach to characterize interactions between tuberculosis and diabetes mellitus. iScience, 27(3), 109135.

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