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Mouse igg2b isotype control 133303

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Mouse IgG2B isotype control (133303) is a laboratory reagent used as a control in immunoassays and flow cytometry experiments. It is a purified mouse monoclonal antibody of the IgG2B isotype that does not bind to any known antigenic targets.

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Lab products found in correlation

Monensin, by Merck Group (2 mentions) 2 4 dinitrofluorobenzene dnfb 2 4 dinitrobenzenesulphonic acid dnbs trypsin from bovine pancreas, by Merck Group (2 mentions) Recombinant murine il 2 i0523, by Merck Group (2 mentions) Anti human nkg2d 1d11, by BioLegend (2 mentions) Anti human cd56 hcd56, by BioLegend (2 mentions) Perm wash buffer 554723, by BD (2 mentions) Stempro accutase a11105 01, by Thermo Fisher Scientific (2 mentions) Efluor 780, by Thermo Fisher Scientific (2 mentions) Ionomycin, by Merck Group (2 mentions) Phorbol 12 myristate 13 acetate, by Merck Group (2 mentions)

Close spelling variants of this product

IgG2b isotype control IgG2B isotype Isotype control Mouse IgG2B IgG2B

2 protocols using mouse igg2b isotype control 133303

1

Quantifying Immune Cell Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
2,4-dinitrofluorobenzene (DNFB), 2,4-dinitrobenzenesulphonic acid (DNBS), trypsin from bovine pancreas, phorbol 12-myristate 13-acetate (PMA), ionomycin, monensin and recombinant murine IL-2 (I0523) were purchased from Sigma Aldrich (Brøndby, Denmark). Anti-mouse-NKG2D (C7), anti-mouse-NKG2D (CX5) and fixable viability dye eFluor780 were purchased from eBioscience (San Diego, CA, USA). Anti-human-CD8 (RPA-T8), anti-human-CD4 (OKT4), anti-human-CD3 (OKT3), anti-human-NKG2D (1D11) and anti-human-CD56 (HCD56) were purchased from Bio-Legend (San Diego, CA, USA). Anti-mouse-CD16/CD32 (2.4G2), anti-mouse-γδ TCR (GL3), anti-mouse-Vγ3 (536), anti-mouse-CD3ε (145-2C11), anti-mouse-IFN-γ (XMG1.2), anti-human γδ TCR (B1.1), anti-human/mouse CLA (HECA-452), streptavidin-APC-Cy7 (554063), Cytofix/Cytoperm (554722) and Perm/Wash buffer (554723) were all purchased from BD Biosciences (Albertslund, DK). Anti-mouse-Rae-1 (237104), anti-mouse-H60 (205326), anti-mouse-Mult-1 (186107), antimouse-NKG2D (191004), anti-human-MICA (159227), anti-human-MICB (236511) and mouse IgG2B isotype control (133303) were purchased from R&D Systems (Abingdon, UK). StemPro Accutase (A11105-01) was purchased from Life Technologies (Nærum, DK).
Nielsen M.M., Dyring-Andersen B., Schmidt J.D., Witherden D., Lovato P., Woetmann A., Ødum N., Poulsen S.S., Havran W.L., Geisler C, & Bonefeld C.M. (2015). NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity. The Journal of investigative dermatology, 135(5), 1311-1319.
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2

Quantifying Immune Cell Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
2,4-dinitrofluorobenzene (DNFB), 2,4-dinitrobenzenesulphonic acid (DNBS), trypsin from bovine pancreas, phorbol 12-myristate 13-acetate (PMA), ionomycin, monensin and recombinant murine IL-2 (I0523) were purchased from Sigma Aldrich (Brøndby, Denmark). Anti-mouse-NKG2D (C7), anti-mouse-NKG2D (CX5) and fixable viability dye eFluor780 were purchased from eBioscience (San Diego, CA, USA). Anti-human-CD8 (RPA-T8), anti-human-CD4 (OKT4), anti-human-CD3 (OKT3), anti-human-NKG2D (1D11) and anti-human-CD56 (HCD56) were purchased from Bio-Legend (San Diego, CA, USA). Anti-mouse-CD16/CD32 (2.4G2), anti-mouse-γδ TCR (GL3), anti-mouse-Vγ3 (536), anti-mouse-CD3ε (145-2C11), anti-mouse-IFN-γ (XMG1.2), anti-human γδ TCR (B1.1), anti-human/mouse CLA (HECA-452), streptavidin-APC-Cy7 (554063), Cytofix/Cytoperm (554722) and Perm/Wash buffer (554723) were all purchased from BD Biosciences (Albertslund, DK). Anti-mouse-Rae-1 (237104), anti-mouse-H60 (205326), anti-mouse-Mult-1 (186107), antimouse-NKG2D (191004), anti-human-MICA (159227), anti-human-MICB (236511) and mouse IgG2B isotype control (133303) were purchased from R&D Systems (Abingdon, UK). StemPro Accutase (A11105-01) was purchased from Life Technologies (Nærum, DK).
Nielsen M.M., Dyring-Andersen B., Schmidt J.D., Witherden D., Lovato P., Woetmann A., Ødum N., Poulsen S.S., Havran W.L., Geisler C, & Bonefeld C.M. (2015). NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity. The Journal of investigative dermatology, 135(5), 1311-1319.
+ Open protocol
+ Expand

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