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Anti aqp2 antibody

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The Anti-AQP2 antibody is a laboratory reagent used for the detection and analysis of the aquaporin-2 (AQP2) protein. AQP2 is a water channel protein involved in water reabsorption in the kidney. This antibody can be used in various research applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the expression and localization of AQP2 in biological samples.

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Lab products found in correlation

Alexa fluor 555 donkey anti goat igg, by Thermo Fisher Scientific (2 mentions) Anti gfp alexa fluor 488 conjugated antibody, by Thermo Fisher Scientific (2 mentions) Anti c myc antibody, by Abcam (2 mentions) Anti gapdh antibody, by Cell Signaling Technology (2 mentions) Dba rhodamine, by Vector Laboratories (2 mentions) Lectins lta fitc, by Vector Laboratories (2 mentions) 3 3 diaminobenzidine (dab), by Agilent Technologies (2 mentions) Streptavidin horseradish peroxidase, by Biocare Medical (2 mentions) Goat anti rabbit biotinylated igg, by Biocare Medical (2 mentions) Mayer hematoxylin, by Agilent Technologies (2 mentions) Metamorph, by Molecular Devices (2 mentions)

Close spelling variants of this product

Anti-AQP2 (2 citations)

5 protocols using anti aqp2 antibody

1

Antibody Identification and Characterization

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Anti-GAPDH antibody was purchased from Cell Signaling Technologies (# 2118). Anti-AQP2 antibody was purchased from Santa Cruz Biotechnologies (# SC9882). Alexa Fluor 488 conjugated anti-GFP antibody was obtained from Life Technologies (# A21311). Polyclonal rabbit anti-cystin antibody (70,053) was generated in our lab and described previously19 (link). Rabbit monoclonal anti-c-Myc antibody was purchased from Abcam (# ab32072). Goat anti-rabbit HRP conjugated secondary antibody was purchased from American Qualex Solution Products (# A102PS). Donkey anti-Goat IgG Alexa Fluor 555 was obtained from Life Technologies (# A21432). Lectins LTA-FITC (# W0909) and DBA-Rhodamine (# Y0828) were obtained from Vector Laboratories.
Yang C., Harafuji N., O’Connor A.K., Kesterson R.A., Watts J.A., Majmundar A.J., Braun D.A., Lek M., Laricchia K.M., Fathy H.M., Mane S., Shril S., Hildebrandt F, & Guay-Woodford L.M. (2021). Cystin genetic variants cause autosomal recessive polycystic kidney disease associated with altered Myc expression. Scientific Reports, 11, 18274.
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2

Kidney Protein Localization via Immunofluorescence

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IHC was performed as previously described.12 (link) Rabbit polyclonal antibodies were used to label RNase 7 (1:50, Sigma-Aldrich) and IR (1:50, Abcam, Cambridge, MA). Triple label immunofluorescence was performed to localize IR expression in the kidney. All sections were prepared as previously described.12 (link) Sections were labeled for principal cells with a goat polyclonal anti-AQP-2 antibody (1:500, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and intercalated cells with a chicken polyclonal anti-V-ATPase E1 subunit antibody (1:2,000, Sigma-Aldrich) or a mouse monoclonal anti-AE1 antibody (1:2,000, kindly provided by M. Jennings).
Eichler T., Becknell B., Easterling R.S., Ingraham S.E., Cohen D.M., Schwaderer A., Hains D.S., Li B., Cohen A., Metheny J., Trindandapani S, & Spencer J.D. (2016). Insulin and the PI3K/AKT Signaling Pathway Regulate Ribonuclease 7 Expression in the Human Urinary Tract. Kidney international, 90(3), 568-579.
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3

Antibody Sources and Fluorescent Lectin Labeling

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Anti-GAPDH antibody was purchased from Cell Signaling Technologies (# 2118). Anti-AQP2 antibody was purchased from Santa Cruz Biotechnologies (# SC9882). Alexa Fluor 488 conjugated anti-GFP antibody was obtained from Life Technologies (# A21311). Polyclonal rabbit anti-cystin antibody (70053) was generated in our lab and described previously. 18 Rabbit monoclonal anti-c-Myc antibody was purchased from Abcam (# ab32072). Goat anti-rabbit HRP conjugated secondary antibody was purchased from American Qualex Solution Products (# A102PS). Donkey anti-Goat IgG Alexa Fluor 555 was obtained from Life Technologies (# A21432).
Lectins LTA-FITC (# W0909) and DBA-Rhodamine (# Y0828) were obtained from Vector Laboratories.
Yang C., O’Connor A.K., Kesterson R.A., Watts J.A., Majmundar A.J., Braun D.A., Lek M., Laricchia K.M., Fathy H., Shril S., Hildebrandt F., & Guay‐Woodford L.M. (2020). Cystin gene mutations cause autosomal recessive polycystic kidney disease associated with alteredMycexpression.
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4

Histochemical Analysis of Kidney Cysts

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Isolated kidneys were fixed in 40% Formalin and routinely embedded, cut at 4 μm slices, dried and deparaffinized for subsequent histochemistry. Hematoxylin and Eosin (H&E) staining was used to assess kidney morphology. The kidney slices were scanned with Nikon (Melville, NY) Super CoolScan 9000 interfaced with NikonScan 4 software. Further analysis of cyst area was performed using color threshholding method using Metamorph (Molecular Devices, Sunnyvale, CA) software as previously described (33 (link)). For immunohistochemistry, tissue sections were incubated with anti-β-ENaC antibodies (StressMarq, Victoria, Canada) or anti-AQP2 antibodies (Santa Cruz Biotechnology, Dallas, TX). Secondary detection was performed with goat anti-rabbit biotinylated IgG (Biocare, Concord, CA) followed by streptavidin horseradish peroxidase (Biocare) and visualized with DAB (DAKO). All slides were counterstained with a Mayer hematoxylin (DAKO, Carpinteria, CA), dehydrated, and mounted with permanent mounting media (Sakura, Torrance, CA).
Captured images were analyzed with ImageJ software (NIH, Bethesda, MD) to measure signal intensities (arbitrary units, ranged from 0 to 255) in tubules and cysts. Intensities from 7–18 individual tubules or cysts were collected each animal; total number of animals per group was three.
Pavlov T.S., Levchenko V., Ilatovskaya D.V., Palygin O, & Staruschenko A. (2014). Impaired epithelial Na+ channels activity contributes to cystogenesis and development of autosomal recessive polycystic kidney disease in PCK rats. Pediatric research, 77(0), 64-69.
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5

Histochemical Analysis of Kidney Cysts

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Isolated kidneys were fixed in 40% Formalin and routinely embedded, cut at 4 μm slices, dried and deparaffinized for subsequent histochemistry. Hematoxylin and Eosin (H&E) staining was used to assess kidney morphology. The kidney slices were scanned with Nikon (Melville, NY) Super CoolScan 9000 interfaced with NikonScan 4 software. Further analysis of cyst area was performed using color threshholding method using Metamorph (Molecular Devices, Sunnyvale, CA) software as previously described (33 (link)). For immunohistochemistry, tissue sections were incubated with anti-β-ENaC antibodies (StressMarq, Victoria, Canada) or anti-AQP2 antibodies (Santa Cruz Biotechnology, Dallas, TX). Secondary detection was performed with goat anti-rabbit biotinylated IgG (Biocare, Concord, CA) followed by streptavidin horseradish peroxidase (Biocare) and visualized with DAB (DAKO). All slides were counterstained with a Mayer hematoxylin (DAKO, Carpinteria, CA), dehydrated, and mounted with permanent mounting media (Sakura, Torrance, CA).
Captured images were analyzed with ImageJ software (NIH, Bethesda, MD) to measure signal intensities (arbitrary units, ranged from 0 to 255) in tubules and cysts. Intensities from 7–18 individual tubules or cysts were collected each animal; total number of animals per group was three.
Pavlov T.S., Levchenko V., Ilatovskaya D.V., Palygin O, & Staruschenko A. (2014). Impaired epithelial Na+ channels activity contributes to cystogenesis and development of autosomal recessive polycystic kidney disease in PCK rats. Pediatric research, 77(0), 64-69.
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