Glyceraldehyde 3 phosphate dehydrogenase gapdh antibody

Fetal bovine serum (FBS) and Roswell Park Memorial Institute (RPMI) 1640 medium were purchased from Gibco (Grand Island, NY, USA). Penicillin-streptomycin and trypsin-ethylenediaminetetraacetic acid (EDTA) were purchased from Hyclone (Logan, UT, USA). Dextrose, sodium bicarbonate, 4-(2-Hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES), dimethyl sulfoxide (DMSO), and thiazolyl blue tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bicinchoninic acid (BCA) kit and SuperSignal^TM west Femto chemiluminescent substrate were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Antibodies against Smad2/3 and kidney injury molecule-1 (KIM-1) were purchased from Cell Signaling (Denver, MA, USA). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was obtained from Millipore (Temecula, CA, USA). Antibodies against AhR, hypoxia-inducible factor 1-alpha (HIF-1α), epithelial cadherin (E-cad), fibronectin (FN), and Bcl-2-associated X protein (Bax), caspase-3 were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). AhR siRNA (5′-GUGACUUGUACAGCAUAAUTT-3′) was purchased from GenePharma (Shanghai, China), Smad2/3 siRNA (sc-37238) was purchased from Santacruz Biotechnology (Santacruz, CA, USA), and HIF-1α siRNA (SASI_HS02_00332063) was purchased Sigma-Aldrich (St. Louis, MO, USA).

OTA was supplied by Cfm Oskar Tropitzsch (Marktredwitz, Germany). Dulbecco′s modified Eagle′s medium (DMEM) and Opti-MEM were from Life Technologies (Grand Island, NY, USA). Penicillin-streptomycin, fetal bovine serum (FBS), and trypsin were purchased from Hyclone (Logan, UT, USA). The bicinchoninic acid (BCA) protein assay kit was purchased from Thermo Scientific (Rockford, IL, USA). In addition, MTT, dimethyl sulfoxide (DMSO), GSH, 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB), glutathione reductase from baker′s yeast (GR), β-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (β-NADPH), 2′,7′-dichlorodihydrofluorescein diacetate (DCFA-DH), 2,3-diaminonaphthalene (DAN), and nitric oxide (NO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The enhanced chemiluminescence (ECL) detection kit was purchased from Abclon (Seoul, South Korea). All of the antibodies used in the experiments were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was provided by Millipore (Termecular, CA, USA).