Miracle attenuated total reflectance attachment

Dry neat (non-drug-loaded) and TM-loaded pSB-co-pVP networks were studied by using ATR-IR spectroscopy. The regime of the attenuated total reflectance of the IRAffinity-1 Shimadzu Fourier Transform Infrared spectrophotometer with the MIRacle Attenuated Total Reflectance Attachment, Kyoto, Japan was used.

The Fourier-transform infrared spectra (FTIR) of pure silibinin, pure PEGylated tert-octylcalix[8]arenes, their physical mixtures, and lyophilized inclusion complexes were measured in the range of 400–4000 cm⁻¹ on an IRAffinity-1 FTIR spectrophotometer with a MIRacle Attenuated Total Reflectance Attachment (Shimadzu, Kyoto, Japan). The samples were analyzed in attenuated total internal reflection absorbance mode, with an aperture diameter of 3 mm and a spectral resolution of 1 cm⁻¹. For an optimal signal-to-noise ratio, 50 scans were averaged per sample spectrum. All the spectra were normalized thereafter.

The interactions of organic molecules (curcumin, capsaicin) with the surface groups of the parent and Ag-containing silica particles were studied with the attenuated total reflection infrared method. ATR-FTIR spectra were recorded by means of IRAffinity-1 Fourier Transform Infrared spectrophotometer (Shimadzu Europa GmbH, Duisburg, Germany) with MIRacle Attenuated Total Reflectance Attachment. The instrument is equipped with a temperature-controlled, high-sensitivity DLATGS detector and ATR attachment with a KRS-5 prism, both purchased from Shimadzu Europa GmbH (Duisburg, Germany). For each sample, 50 scans with 4 cm⁻¹ resolution were applied, and the spectral data wereprocessed with an IR solution software.