C305a gal4

Manufactured by Bloomington Drosophila Stock Center

The C305a-Gal4 is a genetic tool used in Drosophila research. It is a Gal4 driver line that directs the expression of target genes in a specific subset of neurons in the adult Drosophila brain.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using c305a gal4

Genetics of Drosophila Learning Behavior

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following strains were used: y,w and CS were used as the control; y, w, dlg^XI-2; y, w, dlgS97⁵; y, w, dlgA^40.2; (Bloomington Stock Center, Bloomington, IN, USA). All of the strains were backcrossed six-fold with Canton-S to standardize the genetic background (Cantonization). For the adult associative olfactory learning experiments, the strains used were: W(CS); Ok107-GAL4 (kind gift from Dr. Jorge Campusano); UAS-RNAi-dlg (Vienna Drosophila Resource Center, AT); UAS-dsRNA-dlg1; Orco-GAL4; GH146-GAL4; Tenm-GAL4;117y-GAL4; C305A-GAL4; Elav-GAL4 (Bloomington Stock Center, Bloomington, IN); Ok107-GAL4; UAS-DlgS97 (Kind gift from Dr. U. Thomas). For the dNR1 and dNR2 overexpression, UAS-GFP-CD8-dNR1 and UAS-GFP-CD8-dNR2 flies were used. All of the flies were grown at 22 °C.

Bertin F., Moya-Alvarado G., Quiroz-Manríquez E., Ibacache A., Köhler-Solis A., Oliva C, & Sierralta J. (2022). Dlg Is Required for Short-Term Memory and Interacts with NMDAR in the Drosophila Brain. International Journal of Molecular Sciences, 23(16), 9187.

+ Open protocol

+ Expand

Drosophila Rearing and Genetics Procedures

Check if the same lab product or an alternative is used in the 5 most similar protocols

Drosophila melanogaster flies were raised on standard medium (water, cornmeal, soy bean, agar, molasses, yeast, preservative methyl-4-hydroxybenzoate) at 25 °C, 60% humidity and 14 h/10 h light–dark cycles. Canton-S (CS) served as wild-type Drosophila strain in this study. The following colleagues kindly provided additional stocks: D. Armstrong (189Y-GAL4), M. Heisenberg (wild-type Canton-S, rut¹, and dnc¹), T.J. Hirsh (Trh493-GAL4), Y. Rao (5HT-1A-GAL4), H. Scholz (UAS-TNT), P. Shen (UAS-npf), H. Tanimoto (H24-GAL4, NP1131-GAL4 and mb247-GAL4; Tub-GAL80^ts), and T. Zars (mb247-GAL4). The following lines were obtained from the Bloomington Stock Center: Or83b-GAL4 (#2681), c305a-GAL4 (#30829), c739-GAL4(#7362), npf-GAL4 (#25681), 5HT-1B-GAL4 (#27637), UAS-NaChBac (#9469), UAS-5HT-1A^RNAi (#25834 and #33885), UAS-5HT-1B^RNAi (#27634 and #54006), UAS-CD4::tdTomato (#35837) and UAS-mCD8::GFP (#5130).

Ries A.S., Hermanns T., Poeck B, & Strauss R. (2017). Serotonin modulates a depression-like state in Drosophila responsive to lithium treatment. Nature Communications, 8, 15738.

+ Open protocol

+ Expand

Drosophila Husbandry and Genetic Tools

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flies (Drosophila melanogaster) were generally kept at 25°C and subjected to a 12 h light – 12 h dark cycle. A cornmeal medium supplemented with yeast, fructose and molasses was used to rear the flies. Canton-S was used as wild-type (courtesy of R. Stocker). c739-Gal4 was obtained from Hiromu Tanimoto (Tohoku University) and OK107-Gal4 (106098) was received from Kyoto stock center. nSyb-Gal4 (51635), c305a-Gal4 (30829), 5-HTR1B-Gal4 (27636), GMR58E02-Gal4 (41347), G0239-Gal4 (12639), tubGal80^ts (7019), nos-Cas9 (54591) and UAS-FLP (55804, 55806) were obtained from Bloomington stock center.

Widmer Y.F., Fritsch C., Jungo M.M., Almeida S., Egger B, & Sprecher S.G. (2018). Multiple neurons encode CrebB dependent appetitive long-term memory in the mushroom body circuit. eLife, 7, e39196.

+ Open protocol

+ Expand

Genetic Manipulation of Plum in Drosophila

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The plum^Δ1 allele and the following Plum transgenes: UAS-Plum::Flag, UAS-Plum^Δcyt::Flag, UAS-Plum^ΔFNIII(1-5)::Flag, UAS-Plum^ΔIG1-4::Flag, UAS-Plum^ΔIg1-3::Flag, UAS-Plum^ΔIg4::Flag, UAS-Plum^ECD::Flag and UAS-Plum^ECD::TM-CD8::Flag were previously described (Yu et al., 2013 (link)). G0050-Gal4 was kindly provided by A.S. Chiang. UAS-Myo RNAi (31200 and 31114), GMR71G10-Gal4, GMR44E04-Gal4 (originally made as part of the FlyLight project at the Janelia farm, Rubin lab), c305a-Gal4, c155-Gal4, Repo-Gal4 and UAS-Plum RNAi (TRiP.HMC05055, originally made by DRSC) were obtained from the Bloomington Drosophila Stock Center (BDSC).

Marmor-Kollet N., Gutman I., Issman-Zecharya N, & Schuldiner O. (2019). Glial Derived TGF-β Instructs Axon Midline Stopping. Frontiers in Molecular Neuroscience, 12, 232.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!