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Bx430

Manufactured by Bio-Techne
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Sourced in United States

The BX430 is a high-quality microscope designed for laboratory use. It features advanced optics and illumination capabilities to provide clear and detailed images of specimens. The core function of the BX430 is to enable comprehensive visual examination and analysis of samples.

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Lab products found in correlation

Bzatp, by Merck Group (3 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Adenosine triphosphate (atp), by Merck Group (2 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (2 mentions) Bapta am, by Thermo Fisher Scientific (2 mentions) Yo pro 1, by Thermo Fisher Scientific (2 mentions) Yoyo 1, by Thermo Fisher Scientific (2 mentions) Phrodo red e coli bioparticles conjugate, by Thermo Fisher Scientific (2 mentions) Tannic acid, by Merck Group (2 mentions) Nigericin, by Merck Group (2 mentions) 4 4 diisothiocyano 2 2 stilbenedisulfonic acid dids, by Merck Group (2 mentions) 10panx inhibitory peptide, by Bio-Techne (2 mentions) A804598, by Bio-Techne (2 mentions) Lucifer yellow dilithium salt, by Merck Group (2 mentions) Ar c 118925xx, by Bio-Techne (2 mentions) Mrs2500, by Bio-Techne (2 mentions) 5 bdbd, by Bio-Techne (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Ionomycin, by Merck Group (1 mentions)

5 protocols using bx430

1

Investigating Purinergic Signaling Mechanisms

Cited 1 time
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RPMI 1640, penicillin and streptomycin were purchased from Gibco by Life Technologies (Waltham, MA, USA). ATP, BzATP, EDTA, lipopolysaccharides from E. coli O55:B5 (LPS; L2880), dimethyl sulfoxide (DMSO), Na gluconate, K gluconate, Lucifer yellow dilithium salt, 5 (6 (link))-carboxyfluorescein, cabenoxolone, tannic acid, 4,4’-Diisothiocyano-2,2’-stilbenedisulfonic acid (DIDS), CaCCinh-A01 (A01), 5-Nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), nigericin, ionomycin, MCC950, and Melittin (MEL) were purchased from Millipore Sigma (St. Louis, MO, USA). MQAE, BAPTA-AM, YO-PRO-1, YOYO-1, pHrodo Red E. coli BioParticles Conjugate, and 35 mm Nunclon surface dishes were purchased from Invitrogen/ThermoFisher (Carlsbad, CA, USA). A804598, BX430, 10Panx inhibitory peptide, and Y-27632 were purchased from Tocris (Minneapolis, MN, USA). N-(p-amylcinnamoyl) Anthranilic Acid (ACA), Bromoenol lactone (BEL), SB-203580 (SB), PP2, IAA-94, and Ac-YVAD-CMK (YVAD) were purchased from Cayman Chemical (Ann Arbor, Michigan, USA).
Janks L., Sprague R.S, & Egan T.M. (2018). ATP-gated P2X7 receptors require chloride channels to promote inflammation in human macrophages. Journal of immunology (Baltimore, Md. : 1950), 202(3), 883-898.
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2

Calcium Signaling Pathway Modulation

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The following reagents were used: PSB-12062 and ATP (Sigma-Aldrich, USA), A438079 (Abcam, UK), 5-BDBD, MRS-2578, BX430, Ivermectin, Ro0437626, NF340, MRS-2211, Arc-118925xx, MRS2500, U-73122 (all from Tocris, USA), recombinant human GM-CSF (rhuGM-CSF) (Peprotech, USA) and Fura-2AM (TefLabs, USA).
Layhadi J.A., Turner J., Crossman D, & Fountain S.J. (2017). ATP evokes Ca2+ responses and CXCL5 secretion via P2X4 receptor activation in human monocyte-derived macrophages. Journal of immunology (Baltimore, Md. : 1950), 200(3), 1159-1168.
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3

Purified Nucleotides and Antagonists Preparation

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ATP (≥ 99% purity; Abcam), γ-[(propargyl)-imido]-ATP (≥ 95% purity; Sigma), CTP (≥ 95% purity; Sigma), 2-MeSATP (≥ 98% purity; Tocris), BzATP (≥ 93% purity; Sigma), ATPγS (≥ 90% purity; Tocris), Ap4A (≥ 95% purity; Sigma), suramin (≥ 98% purity; Sigma), PPADS (≥ 98% purity; Sigma) and TNP-ATP (≥ 95% purity; Tocris) were all dissolved in water. 5-BDBD (≥ 99% purity; Tocris), BX-430 (≥ 99% purity; Tocris), PSB-12062 (≥ 98% purity; Sigma) and BAY-1797 (≥ 98% purity; Cambridge Biosciences) were dissolved in dimethyl sulfoxide (DMSO).
Fortuny-Gomez A, & Fountain S.J. (2024). Pharmacological differences between human and mouse P2X4 receptor explored using old and new tools. Purinergic signalling.
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4

Pharmacological Agents for Ion Channel and Purine Receptor Modulation

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BPTU, AR-C 118925XX, Bx430, 5-BDBD, MRS2179, MRS2279, MRS2500, 10-Panx, suramin, TAT-Gap19, PPADS (Pyridoxalphosphate-6-azophenyl-2’,4’-disulfonic acid), NOC-7, prostaglandin E2, ARL 67156, and ω-agatoxin were purchased from Tocris Bioscience. Apyrase grade VI (catalog no. A6410) and apyrase grade VII (catalog no. A6535) were purchased from Sigma-Aldrich. 18β-glycyrrhetinic acid and carbenoxolone were purchased from Santa Cruz Biotechnology.
Chang-Graham A.L., Perry J.L., Engevik M.A., Engevik K.A., Scribano F.J., Gebert J.T., Danhof H.A., Nelson J.C., Kellen J.S., Strtak A.C., Sastri N.P., Estes M.K., Britton R.A., Versalovic J, & Hyser J.M. (2020). Rotavirus induces intercellular calcium waves through ADP signaling. Science (New York, N.Y.), 370(6519), eabc3621.
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5

Detailed Cytotoxicity Assays Protocol

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Dulbecco’s modified Eagle’s medium (DMEM), RPMI 1640, fetal bovine serum (FBS), penicillin, and streptomycin were purchased from Gibco by Life Technologies (Waltham, MA, USA). ATP, BzATP, EDTA, ethidium bromide, lipopolysaccharides from E. coli O55:B5 (LPS; L2880), dimethyl sulfoxide (DMSO), Lucifer yellow dilithium salt, 5(6)-carboxyfluorescein, carbenoxolone, probenecid, tannic acid, 4,4′-diisothiocyano-2,2′-stilbenedisulfonic acid (DIDS), and nigericin were purchased from Millipore-Sigma (St. Louis, MO, USA). BAPTA-AM, YO-PRO-1, YOYO-1, Fluo-4-AM, pHrodo Red E. coli BioParticles Conjugate, and pHrodo Green AM were purchased from Invitrogen/ThermoFisher (Carlsbad, CA, USA). A438079, A804598, BX430, and 10Panx inhibitory peptide were purchased from Tocris (Minneapolis, MN, USA). Complete growth differentiated media with serum (E37089-01-S), extracellular matrix-coated T25 or T75 culture flasks (E37089-01-T25,T75), and Xeno-free cell dissociation media (M37001-02CM) were obtained from Celprogen (Torrance, CA, USA).
Janks L., Sharma C.V, & Egan T.M. (2018). A central role for P2X7 receptors in human microglia. Journal of Neuroinflammation, 15, 325.
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