T cell mitogen concanavalin a con a

RPMI-1640 was purchased from Gibco (Grand Island, NY, USA). DPPH, Vc, H₂O₂, pyrogallic acid, T-cell mitogen Concanavalin A (Con A), bovine serum albumin, and cellulose sacks were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Filter membrane was purchased from Millipore Co. (Billerica, MA, USA). 3-(4,5-Dimethylthia-zol-2-yl)-2,5-dipheny-ltetrazoliumbromide (MTT) was purchased from Amresco Co. Yeast β-1,3-d-glucan standard was purchased from Putus Macromolecular Sci. & Tech. Ltd. (Wuhan, China). Dimethyl sulfoxide (DMSO), phenol, trichloroacetic acid, Coomassie brilliant blue G250, NaOH, acetic acid, urea, and H₂SO₄ were purchased from Yixin Institute of Chemical Engineering in Jiangsu. Assay kits for the MDA, CAT, SOD, and GSH-Px were purchased from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Anti-CD3-PerCP, anti-CD4-PE, and anti-CD8-FITC antibodies were purchased from BD eBiosciences (San Diego, CA, USA). IL-2 and IFN-γ kits were purchased from R&D systems (Minneapolis, MN, USA). All chemicals used in the experiments were analytical grade.

FITC-conjugated anti-CD3, APC-conjugated anti-CD4, APC-conjugated anti-CD8, and their corresponding isotype controls were purchased from eBioscience (San Diego, CA). PE-Cy5-conjugated anti-CD4, PE-Cy5-conjugated anti-CD8, FITC-conjugated anti-CD44, and their corresponding isotype controls, were obtained from BioLegend (San Diego, CA). PE-conjugated anti-CD45RB, PE-conjugated anti-Fas, and their corresponding isotype controls were purchased from BD Bioscience (San Jose, CA). FITC-conjugated anti-CD28 and its corresponding isotype control were purchased from Serotec (Oxfordshire, UK). Rabbit polyclonal primary anti-Ki-67 and its isotype control were purchased from Abcam (Cambridge, UK) and Santa Cruz Biotechnology (Santa Cruz, CA), respectively. PerCP-conjugated donkey anti-rabbit IgG antibody was obtained from Santa Cruz Biotechnology. T cell mitogen concanavalin A (Con A) was obtained from Sigma (St. Louis, MO).