For the diagnosis of IC and IA, detection of mannan (Man) or galactomannan (GM) and (1,3)-β-d -glucan (BDG) was performed in the participating centers during routine patient screening or in the Lille Clinical Mycology Laboratory if they had not been tested previously.
BDG was measured using a Fungitell kit (Associates of Cape Cod Inc., Falmouth, MA, USA) following the manufacturer’s instructions. The recommended cutoff of 80 pg/ml was used to determine clinical relevance.
Measurement of serum Man and GM was performed using a Platelia Candida Ag Plus test and Platelia Aspergillus Ag test (Bio-Rad, Marnes la Coquette, France), respectively, according to the manufacturer's instructions. The recommended cutoffs of a concentration of 62.5 pg/ml and an index of ≥0.5, respectively, were used.
For the diagnosis of MM, quantitative real-time PCR (qPCR) was performed as described previously (24 (link), 31 (link)).
BDG was measured using a Fungitell kit (Associates of Cape Cod Inc., Falmouth, MA, USA) following the manufacturer’s instructions. The recommended cutoff of 80 pg/ml was used to determine clinical relevance.
Measurement of serum Man and GM was performed using a Platelia Candida Ag Plus test and Platelia Aspergillus Ag test (Bio-Rad, Marnes la Coquette, France), respectively, according to the manufacturer's instructions. The recommended cutoffs of a concentration of 62.5 pg/ml and an index of ≥0.5, respectively, were used.
For the diagnosis of MM, quantitative real-time PCR (qPCR) was performed as described previously (24 (link), 31 (link)).